UACC-62 Cell Avalanche® Transfection Reagent

UACC-62 Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting UACC-62 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for UACC-62 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

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$714.00$1,264.00

 
Description

Cells to transfect:

Cell Line Name: UACC-62, UACC.62

NCI60, NCI-60, UACC.62

Species: Homo sapiens

Tissue Type: Skin

Origin: Malignant melanoma

Histology: Melanotic melanoma; non epithelial

Groth Properties: adherent

p53 Status: 1

ECAD Methylation: 43

MDR Function: 8

Cytogenetic Analysis: 3n+/-, Near-triploid 69+/- (58-80)

Institution: University of Arizona

Contributor: Leibovitz, A

UACC-62 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

Transfection reagent features:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Specifically optimized to deliver nucleic acids into UACC-62 cells
  • Highest efficiency to ensure experimental success
  • Extremely gentle to cells
  • Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Simplest and straightforward protocols to Increase experimental throughput
  • Compatible with serum
  • Suitable for Reverse Transfection
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Developed and manufactured by EZ Biosystems

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on UACC-62 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for UACC-62 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this UACC-62 Cell Avalanche® Transfection Reagent.

 

 

 

 

 

 

 

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on UACC-62 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for UACC-62 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this UACC-62 Cell Avalanche® Transfection Reagent.

For Other Cells

UACC-62 Cell Avalanche® Transfection Reagent (human melanoma cell) can also be used on the following cells with high transfection efficiencies.

LOX-IMVI Cell

M14 Cell

SK-MEL-5 Cell

UACC-257 Cell

A-375 Cell

SK-MEL-28 Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Adherent

Cell Type

Others

Disease

Cancer

Names starting from

U

Primary/Cell Line

Cell Line

Product Sizes

0.5 ml, 1.5 ml

Species

Human

Tissue Sources

Melanoma

Subcategories

Cell Type/Cell Line Specific

Documents



Protocols


MSDS



Citations or Feedback

Testimonials and Citations

 
Description

Cells to transfect:

Cell Line Name: UACC-62, UACC.62

NCI60, NCI-60, UACC.62

Species: Homo sapiens

Tissue Type: Skin

Origin: Malignant melanoma

Histology: Melanotic melanoma; non epithelial

Groth Properties: adherent

p53 Status: 1

ECAD Methylation: 43

MDR Function: 8

Cytogenetic Analysis: 3n+/-, Near-triploid 69+/- (58-80)

Institution: University of Arizona

Contributor: Leibovitz, A

UACC-62 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

Transfection reagent features:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Specifically optimized to deliver nucleic acids into UACC-62 cells
  • Highest efficiency to ensure experimental success
  • Extremely gentle to cells
  • Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Simplest and straightforward protocols to Increase experimental throughput
  • Compatible with serum
  • Suitable for Reverse Transfection
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Developed and manufactured by EZ Biosystems

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on UACC-62 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for UACC-62 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this UACC-62 Cell Avalanche® Transfection Reagent.

 

 

 

 

 

 

 

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on UACC-62 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for UACC-62 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this UACC-62 Cell Avalanche® Transfection Reagent.

For Other Cells

UACC-62 Cell Avalanche® Transfection Reagent (human melanoma cell) can also be used on the following cells with high transfection efficiencies.

LOX-IMVI Cell

M14 Cell

SK-MEL-5 Cell

UACC-257 Cell

A-375 Cell

SK-MEL-28 Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Adherent

Cell Type

Others

Disease

Cancer

Names starting from

U

Primary/Cell Line

Cell Line

Product Sizes

0.5 ml, 1.5 ml

Species

Human

Tissue Sources

Melanoma

Subcategories

Cell Type/Cell Line Specific

Documents



Protocols


MSDS



Citations or Feedback

Testimonials and Citations

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