NCI-H522 Cell Avalanche® Transfection Reagent

 
Description

Cells to transfect:

Cell Line Name: NCI-H522

Cell Synonyms: NCI60, NCI-60

Organism: Homo sapiens, human

Tissue: lung

Morphology: epithelial

Culture Properties: adherent, single cells and loosely attached clusters

Biosafety Level: 1

Disease: stage 2, adenocarcinoma; non-small cell lung cancer

Age: 58 years

Gender: male

Ethnicity: Caucasian

Comments: The cells carry the K-ras 12 mutation, and there is a mutation in codon 191 of the p53 gene.The cells are not able to synthesize the peptide neuromedin B (NMB) or the gastrin releasing peptide (GRP).The cells stain positive for keratin and vimentin but are negative for neurofilament triplet protein.

Institution: University of Texas Southwestern Medical Center

Contributor: This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lung cancer obtained from a patient prior to therapy. The patient was a smoker (60-pack-year).

NCI-H522 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

Transfection reagent features:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Specifically optimized to deliver nucleic acids into NCI-H522 cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Free of animal-derived components
• Compatible with serum
• Active in serum-containing medium
• Suitable for Reverse Transfection
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NCI-H522 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NCI-H522 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this NCI-H522 Cell Avalanche® Transfection Reagent.

For Other Cells

NCI-H522 Cell Avalanche® Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies.

EKVX Cell

HOP-92 Cell

NCI-H226 Cell

NCI-H322M Cell

A549 Cell

NCI-H23 Cell

NCI-H1975 Cell

Calu-3 Cell

NCI-H358 Cell

293 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

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