EZT-U266-1 Protocol.
Description
Cell to transfect:
Designations: U266B1
Cell Synonyms: : U266, TIB196, human myeloma cell
Organism: Homo sapiens (human)
Tissue: B lymphocyte; plasmacytoma; myeloma
Age: 53 years
Gender: male
Cellular products: immunoglobulin; monoclonal antibody; interleukin 6 (interleukin-6, IL-6)
Isotype: IgE; lambda light chain
Morphology: lymphoblast
Growth properties: suspension
Depositors: N.K. Nilsson
The U266 cell line was established from the peripheral blood of a patient with an IgE myeloma (epsilon2, lambda2). These cells secrete IgE. Immunological studies indicate that the U266 cell line was derived from the same clone of myeloma cells which grew in vivo since the immunoglobulin synthesized in vitro by the cell line is identical to the myeloma protein produced in vivo. U266 cells have been reported to produce human IL-6.
Features of The Transfection Reagent:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Highest efficiency to ensure experimental success
- Extremely gentle to cells
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Deliver single or multiple plasmids
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Compatible with serum
- Suitable for Reverse Transfection
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on U266B1 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for U266B1 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this U266B1 Cell Avalanche Transfection Reagent.
For Other Cells
U266B1 Cell Avalanche® Transfection Reagent (human myeloma cell) can also be used on the following cells with high transfection efficiencies.
Vero Cell
293 Cell
293T/17 Cell
MG-63 Cell
WI-38 Cell
MRC-5 Cell
IMR-90 Cell
COS-7 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype |
Suspension |
| Cell Type |
lymphocyte |
| Disease |
Cancer |
| Names starting from |
U |
| Primary/Cell Line |
Cell Line |
| Product Sizes |
0.5 ml, 1.5 ml |
| Species |
Human |
| Tissue Sources |
Blood |
| Subcategories |
Cell Type/Cell Line Specific |
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