SK-N-SH Cell Avalanche® Transfection Reagent (human neuroblastoma cell)

SK-N-SH Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting SK-N-SH cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for SK-N-SH cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

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$714.00$1,264.00

 
Description

Cell Line Information:

Designations: SK-N-SH

Cell Synonyms: HTB-11, human neuroblastoma cell

Organism: Homo sapiens (human)

Tissue: neuroblastoma; brain; derived from metastatic site: bone marrow

Age: 4 years

Gender: female

Morphology: epithelial

Growth properties: adherent

AntigenExp: Blood Type A; Rh+

Products: plasminogen activator shows increased expression of M-CSF after treatment with amyloid-beta peptide

Depositors: G. Trempe; L.J. Old

The SK-N-SH line was developed by J.L. Biedler. SK-N-SH has been used as a target cell line in cell mediated cytotoxicity assays.

Features of The Transfection Reagent:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Specifically optimized to deliver nucleic acids into SK-N-SH cells
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Suitable for Reverse Transfection
  • Compatible with transfection in any plate formats
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on SK-N-SH cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for SK-N-SH cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this SK-N-SH Cell Avalanche Transfection Reagent.

For Other Cells

SK-N-SH Cell Avalanche® Transfection Reagent (human neuroblastoma cell) can also be used on the following cells with high transfection efficiencies.

LA-N-2

IMR-32

786-O Cell

Caki-1 Cell

MDCK Cell

Vero Cell

DU 145 Cell

PC-3 Cell

AR42J Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Adherent

Cell Type

Epithelial Cell

Disease

Cancer

Names starting from

S

Primary/Cell Line

Cell Line

Product Sizes

0.5 ml, 1.5 ml

Species

Human

Tissue Sources

Neuroblastoma

Subcategories

Cell Type/Cell Line Specific

Documents



Protocols


MSDS



Citations or Feedback

Testimonials and Citations

 
Description

Cell Line Information:

Designations: SK-N-SH

Cell Synonyms: HTB-11, human neuroblastoma cell

Organism: Homo sapiens (human)

Tissue: neuroblastoma; brain; derived from metastatic site: bone marrow

Age: 4 years

Gender: female

Morphology: epithelial

Growth properties: adherent

AntigenExp: Blood Type A; Rh+

Products: plasminogen activator shows increased expression of M-CSF after treatment with amyloid-beta peptide

Depositors: G. Trempe; L.J. Old

The SK-N-SH line was developed by J.L. Biedler. SK-N-SH has been used as a target cell line in cell mediated cytotoxicity assays.

Features of The Transfection Reagent:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Specifically optimized to deliver nucleic acids into SK-N-SH cells
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Suitable for Reverse Transfection
  • Compatible with transfection in any plate formats
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on SK-N-SH cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for SK-N-SH cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this SK-N-SH Cell Avalanche Transfection Reagent.

For Other Cells

SK-N-SH Cell Avalanche® Transfection Reagent (human neuroblastoma cell) can also be used on the following cells with high transfection efficiencies.

LA-N-2

IMR-32

786-O Cell

Caki-1 Cell

MDCK Cell

Vero Cell

DU 145 Cell

PC-3 Cell

AR42J Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Adherent

Cell Type

Epithelial Cell

Disease

Cancer

Names starting from

S

Primary/Cell Line

Cell Line

Product Sizes

0.5 ml, 1.5 ml

Species

Human

Tissue Sources

Neuroblastoma

Subcategories

Cell Type/Cell Line Specific

Documents



Protocols


MSDS



Citations or Feedback

Testimonials and Citations

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