NCI-H226 Cell Avalanche® Transfection Reagent

NCI-H226 Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting NCI-H226 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for NCI-H226 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

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$714.00$1,264.00

 
Description

Cells to transfect:

Cell Line Name: NCI-H226 [H226] (ATCC® CRL-5826®)

Cell Synonyms: NCI60, NCI-60, H226, CRL-5826®

Organism: Homo sapiens, human

Tissue: lung, pleural effusion

Morphology: Epithelial

Culture Properties: adherent

Biosafety Level: 1

Disease: squamous cell carcinoma; mesothelioma

Gender: male

Karyotype: modal number = 47; range = 32-88; del(p25)

Name of Depositor: AF Gazdar, JD Minna

Year of Origin: March, 1980

Institution: University of Texas Southwestern Medical Center

NCI-H226 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

Transfection reagent features:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Unique formulation-maximize transfection performance in NCI-H226 cells
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Active in serum-containing medium
  • Suitable for Reverse Transfection
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NCI-H226 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NCI-H226 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this NCI-H226 Cell Avalanche® Transfection Reagent.

For Other Cells

NCI-H226 Cell Avalanche® Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies.

HOP-92 Cell

NCI-H322M Cell

A549 Cell

NCI-H292 Cell

NCI-H460 Cell

NCI-H23 Cell

NCI-H1975 Cell

NCI-H441 Cell

293 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Adherent

Cell Type

Epithelial Cell

Disease

Cancer

Names starting from

N

Primary/Cell Line

Cell Line

Product Sizes

0.5 ml, 1.5 ml

Species

Human

Tissue Sources

Lung

Subcategories

Cell Type/Cell Line Specific

Documents



Protocols


MSDS



Citations or Feedback

Testimonials and Citations

 
Description

Cells to transfect:

Cell Line Name: NCI-H226 [H226] (ATCC® CRL-5826®)

Cell Synonyms: NCI60, NCI-60, H226, CRL-5826®

Organism: Homo sapiens, human

Tissue: lung, pleural effusion

Morphology: Epithelial

Culture Properties: adherent

Biosafety Level: 1

Disease: squamous cell carcinoma; mesothelioma

Gender: male

Karyotype: modal number = 47; range = 32-88; del(p25)

Name of Depositor: AF Gazdar, JD Minna

Year of Origin: March, 1980

Institution: University of Texas Southwestern Medical Center

NCI-H226 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

Transfection reagent features:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Unique formulation-maximize transfection performance in NCI-H226 cells
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Active in serum-containing medium
  • Suitable for Reverse Transfection
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NCI-H226 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NCI-H226 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this NCI-H226 Cell Avalanche® Transfection Reagent.

For Other Cells

NCI-H226 Cell Avalanche® Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies.

HOP-92 Cell

NCI-H322M Cell

A549 Cell

NCI-H292 Cell

NCI-H460 Cell

NCI-H23 Cell

NCI-H1975 Cell

NCI-H441 Cell

293 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Adherent

Cell Type

Epithelial Cell

Disease

Cancer

Names starting from

N

Primary/Cell Line

Cell Line

Product Sizes

0.5 ml, 1.5 ml

Species

Human

Tissue Sources

Lung

Subcategories

Cell Type/Cell Line Specific

Documents



Protocols


MSDS



Citations or Feedback

Testimonials and Citations

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