SK-BR-3 Cell Avalanche® Transfection Reagent (human breast carcinoma cell)

 
Description

Cell to transfect:

Designations: SK-BR-3

Cell Synonyms: : HTB-30, human breast carcinoma cell

Depositors: G Trempe, LJ Old

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: mammary gland; breast

Disease: adenocarcinoma

Derived from metastatic site: pleural effusion

Isolation date: 1970

Applications: transfection host

Tumorigenic: Yes

Antigen Expression: Blood Type A; Rh+; HLA A11, Bw22(+/-), B40, B18

Age: 43 years

Gender: female

Ethnicity: Caucasian

Comments: The SK-BR-3 cell line overexpresses the HER2/c-erb-2 gene product. Ultrastructural features include microvilli and desmosomes, glycogen granules, large lysosomes, bundles of cytoplasmic fibrils.

Features of The Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Specifically optimized to deliver nucleic acids into SK-BR-3 cells
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Efficient for plasmid DNA/siRNA co-transfection
• Compatible with serum
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on MDA-MB-453 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for MDA-MB-453 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on SK-BR-3 cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as SK-BR-3 Cell Avalanche® Transfection Reagent.

FIG. 2. SK-BR-3 cells were transfected with GFP vector (pEGFP-N3) by using SK-BR-3 Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.

For Other Cells

SK-BR-3 Cell Avalanche® Transfection Reagent (human breast carcinoma cell) can also be used on the following cells with high transfection efficiencies.

4T1 Cell

ZR-75-1 Cell

BT-20 Cell

MDA-MB-453 Cell

HCC1937 Cell

MDA-MB-468 Cell

MDA-MB-231 Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

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