NCI-H292 Cell Avalanche® Transfection Reagent (human lung carcinoma cell)

 
Description

Cell to transfect:

Designations: NCI-H292

Cell Synonyms: CRL-1848, H292, human lung carcinoma cell

VirusSuscept: hepatitis B virus (HBV)

Tumorigenic: yes, in nude mice; tumors histologically resemble the original biopsy specimen and retain mucoepidermoid features

Products: keratin; vimentin

DoublingTime: 48 hrs

Depositors: A.F. Gazdar

Comments: This line was derived from a lymph node metastasis of a pulmonary mucoepidermoid carcinoma. The cells retain their mucoepidermoid characteristics in culture as determined by their ultrastructure and expression of multiple markers of squamous differentiation. The line has been selected as a prototype for transfecting human subgenomic fragments into human cells for studying the role of HBV and its individual genes in the pathogenesis of viral hepatitis and liver cancer. The cells are mucicarmine positive but are negative for neurofilament triplet protein.

Features of The Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Specifically optimized to deliver nucleic acids into NCI-H292 cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Compatible with serum
• Suitable for Reverse Transfection
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on A549 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for A549 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on NCI-H292 cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as NCI-H292 Cell Avalanche Transfection Reagent.

For Other Cells

NCI-H292 Cell Avalanche® Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies.

A549 Cell

NCI-H460 Cell

NCI-H1299 Cell

NCI-H23 Cell

NCI-H1975 Cell

NCI-H441 Cell

Calu-3 Cell

NCI-H358 Cell

293 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

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