MDA-MB-453 Cell Avalanche® Transfection Reagent (human breast carcinoma cell)

 
Description

Cell to transfect:

Designations: MDA-MB-453

Cell Synonyms: : HTB-131, human breast carcinoma cell

Depositors: R Cailleau

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: mammary gland; breast

Disease: metastatic carcinoma

Derived from metastatic site: pericardial effusion

Isolation date: June 10, 1976

Applications: transfection host

Receptors: fibroblast growth factor (FGF), expressed

Tumorigenic: No

Age: 48 years

Gender: female

Ethnicity: Caucasian

Comments: MDA-MB-453 was derived in 1976 by R. Cailleau et al. from an effusion of a 48 year old female patient with metastatic carcinoma of the breast, involving the nodes, brain and both pleural and pericardial cavities. The cells overexpress FGF receptors.

Features of The Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Unique formulation-maximize transfection performance in MDA-MB-453 cells.
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Efficient for plasmid DNA/siRNA co-transfection
• Suitable for Reverse Transfection
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with
• nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on MDA-MB-453 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for MDA-MB-453 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this MDA-MB-453 Cell Avalanche® Transfection Reagent.

FIG. 2. MDA-MB-453 cells were transfected with GFP vector (pEGFP-N3) by using MDA-MB-453 Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.

For Other Cells

MDA-MB-453 Cell Avalanche® Transfection Reagent (human breast carcinoma cell) can also be used on the following cells with high transfection efficiencies.

4T1 Cell

ZR-75-1 Cell

BT-20 Cell

HCC1937 Cell

MDA-MB-468 Cell

SK-BR-3 Cell

MDA-MB-231 Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Documents

Protocols

EZT-MDAM-3 Protocol.

MSDS

EZT-MDAM-3 MSDS

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