Hepa 1-6 Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Hepa 1-6 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Hepa 1-6 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
Comments: This is a derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse.
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Specifically optimized to deliver nucleic acids into Hepa 1-6 cells
Highest efficiency to ensure experimental success
Extremely gentle to cells
0.5 ml is able to transfect about 1000 wells of 24-well plate
Deliver single or multiple plasmids
Efficient for plasmid DNA/siRNA co-transfection
Compatible with serum
Suitable for Reverse Transfection
Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on HEPA 1-6 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for HEPA 1-6 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this HEPA 1-6 Cell Avalanche Transfection Reagent.
FIG. 2. Hepa 1-6 cells were transfected with GFP vector (pEGFP-N3) by using Hepa 1-6 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
Hepa 1-6 Cell Avalanche® Transfection Reagent (mouse hepatoma cell) can also be used on the following cells with high transfection efficiencies.
Hep G2 Cell
Hep 3B Cell
HepG2/C3A Cell
PANC-1 Cell
BxPC-3 Cell
AsPC-1 Cell
CHO-K1 Cell
SK-OV-3 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Zhang, L., Bernardo, K. D., Vickers, T. A., Tian, J., Liang, X. H., & Crooke, S. T. (2022). NAT10 and DDX21 Proteins Interact with RNase H1 and Affect the Performance of Phosphorothioate Oligonucleotides. Nucleic Acid Ther, 32(4), 280-299. doi:10.1089/nat.2021.0107
Comments: This is a derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse.
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Specifically optimized to deliver nucleic acids into Hepa 1-6 cells
Highest efficiency to ensure experimental success
Extremely gentle to cells
0.5 ml is able to transfect about 1000 wells of 24-well plate
Deliver single or multiple plasmids
Efficient for plasmid DNA/siRNA co-transfection
Compatible with serum
Suitable for Reverse Transfection
Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on HEPA 1-6 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for HEPA 1-6 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this HEPA 1-6 Cell Avalanche Transfection Reagent.
FIG. 2. Hepa 1-6 cells were transfected with GFP vector (pEGFP-N3) by using Hepa 1-6 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
Hepa 1-6 Cell Avalanche® Transfection Reagent (mouse hepatoma cell) can also be used on the following cells with high transfection efficiencies.
Hep G2 Cell
Hep 3B Cell
HepG2/C3A Cell
PANC-1 Cell
BxPC-3 Cell
AsPC-1 Cell
CHO-K1 Cell
SK-OV-3 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Zhang, L., Bernardo, K. D., Vickers, T. A., Tian, J., Liang, X. H., & Crooke, S. T. (2022). NAT10 and DDX21 Proteins Interact with RNase H1 and Affect the Performance of Phosphorothioate Oligonucleotides. Nucleic Acid Ther, 32(4), 280-299. doi:10.1089/nat.2021.0107
EZT-HEPA-1 Protocol.
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on HEPA 1-6 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for HEPA 1-6 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this HEPA 1-6 Cell Avalanche Transfection Reagent.
