HCC2998 Cell Avalanche® Transfection Reagent

 
Description

Cells to transfect:

Cell Line Name: HCC2998

Cell Synonyms: NCI60, NCI-60

Tissue Sources: Large intestine; Colon;

Carcinoma; Adenocarcinoma;

Morphology: epithelium

Institution: M.D. Anderson Hospital & Tumor Institute

Contributor: Fidler, IJ

Institution: Developmental Therapeutics Program National Cancer Institute,Frederick,MD 21701

Comments: HCC2998 is a highly differentiated human colon carcinoma cell line, which has been shown to be converted to a poorly differentiated one after expression of a constitutively active phosphatidylinositol 3-kinase (PI3′ kinase). These cells express aberrant sizes of a regulatory subunit of PI3′ kinase, p85alpha, with molecular weights of 50 and 76 kDa at a very low level.

HCC2998 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

Transfection reagent features:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Specifically optimized to deliver nucleic acids into HCC2998 cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Free of animal-derived components
• Active in serum-containing medium
• Suitable for Reverse Transfection
• Compatible with transfection in any plate format
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on HCC2998 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for HCC2998 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this HCC2998 Cell Avalanche® Transfection Reagent.

For Other Cells

HCC2998 Cell Avalanche® Transfection Reagent (human colon adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies.

HCT-15 Cell

KM12 Cell

T84 Cell

DLD-1 Cell

HT-29 Cell

Caco-2 Cell

LoVo Cell

SW480 Cell

293 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

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