EZT-CCRF-1 Protocol.
Description
Cell to transfect:
Designations: CCRF-CEM
Cell Synonyms: CCL-119, NCI60, NCI-60, human leukemia cell
Organism: Homo sapiens (human)
Tissue: leukemia, acute lymphoblastic; peripheral blood; T lymphoblast
Age: 4 years
Gender: female
Ethnicity: caucasian
Morphology: lymphoblast
Growth properties: suspension
Tumorigenic: yes, in syrian hamsters
Reverse Transcriptase: negative
AntigenExp: CD3 B (37%), CD4 (50%), CD5 (95%), CD7 (77%)
Depositor: G.E. Foley
CCRF-CEM is a T lymphoblastoid cell line derived by G.E. Foley, et al. Cells were obtained in November, 1964 from peripheral blood buffy coat of a 4-year-old Caucasian female with acute lymphoblastic leukemia.
CCRF-CEM is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.
Features of The Transfection Reagent:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Specifically optimized to deliver nucleic acids into CCRF-CEM cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Deliver single or multiple plasmids
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Compatible with serum
- Suitable for Reverse Transfection
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on CCRF-CEM cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for CCRF-CEM cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this CCRF-CEM Cell Avalanche Transfection Reagent.
For Other Cells
CCRF-CEM Cell Avalanche® Transfection Reagent (human leukemia cell) can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
MRC-5 Cell
IMR-90 Cell
COS-7 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype | Suspension |
| Cell Type | lymphocyte |
| Disease | Cancer |
| Names starting from | C |
| Primary/Cell Line | Cell Line |
| Product Sizes | 0.5 ml, 1.5 ml |
| Species | Human |
| Tissue Sources | Blood |
| Subcategories | Cell Type/Cell Line Specific |
Documents
Protocols
MSDS
Citations or Feedback
