SK-MEL-5 Cell Avalanche® Transfection Reagent

 
Description

Cells to transfect:

Cell Synonyms: NCI60, NCI-60, HTB-70

Cell Line Name: SK-MEL-5 (ATCC® HTB-70®)

Organism: Homo sapiens, human

Tissue: skin, derived from metastatic axillary node.

Cell Type: Melanoma

Morphology: stellate

Culture Properties: adherent

Biosafety Level: 1

Disease: malignant melanoma

Age: 24 years

Gender: female

Ethnicity: Caucasian

Markers: Blood Type O; Rh+; HLA A2, A11, B40, Bw16

Tumorigenic: Yes

Comments: This is one of a very extensive series of melanoma lines (see also ATCC HTB-71, ATCC HTB-72 and ATCC HTB-73) isolated by T. Takahashi and associates. The lines served as source of target cells for detection of melanoma specific antibody in patients with the disease.

Institution: The Memorial Sloan-Kettering Cancer Center

SK-MEL-5 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

Transfection reagent features:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Unique formulation-maximize transfection performance in SK-MEL-5 cells
• Extremely gentle to cells
• Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Free of animal-derived components
• Active in serum-containing medium
• Suitable for Reverse Transfection
• Compatible with transfection in any plate format
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on SK-MEL-5 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for SK-MEL-5 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this SK-MEL-5 Cell Avalanche® Transfection Reagent.

For Other Cells

SK-MEL-5 Cell Avalanche® Transfection Reagent (human melanoma cell) can also be used on the following cells with high transfection efficiencies.

LOX-IMVI Cell

M14 Cell

SK-MEL-2 Cell

UACC-257 Cell

UACC-62 Cell

A-375 Cell

Vero Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Documents

Protocols

EZT-SME5-1 Protocol.

MSDS

EZT-SME5-1 MSDS

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