EZT-CALU-1 Protocol.
Description
Cell to transfect:
Designations: Calu-3
Cell Synonyms: HTB-55, human lung carcinoma cell
Organism: Homo sapiens (human)
Tissue: lung adenocarcinoma; derived from metastatic site: pleural effusion
Age: 25 years
Gender: male
Ethnicity: Caucasian
Morphology: epithelial
Growth Properties: adherent
Tumorigenic: yes, forms well differentiated grade I adenocarcinoma in nude mice
Antigen Expression: Blood Type A; Rh+
Depositors: J. Fogh
The patient had received prior therapy with cytoxan, bleomycin and adriamycin.
Features of The Transfection Reagent:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Specifically optimized to deliver nucleic acids into Calu-3 cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on A549 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for A549 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on Calu-3 cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as Calu-3 Cell Avalanche Transfection Reagent.
For Other Cells
Calu-3 Cell Avalanche® Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies.
A549 Cell
NCI-H292 Cell
NCI-H460 Cell
NCI-H1299 Cell
NCI-H23 Cell
NCI-H1975 Cell
NCI-H441 Cell
NCI-H358 Cell
293 Cell
Calu-1 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype | Adherent |
| Cell Type | Epithelial Cell |
| Disease | Cancer |
| Names starting from | C |
| Primary/Cell Line | Cell Line |
| Product Sizes | 0.5 ml, 1.5 ml |
| Species | Human |
| Tissue Sources | Lung |
| Subcategories | Cell Type/Cell Line Specific |
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