AR42J Cell Avalanche® Transfection Reagent (rat pancreas tumor cell)

 
Description

Cell to transfect:

Designations: AR42J

Cell Synonyms: : CRL-1492, exocrine, rat pancreas tumor cell

Organism: Rattus norvegicus (rat)

Strain: Wistar

Tissue: pancreas; exocrine; tumor

Morphology: epithelial

Growth properties: adherent

Tumorigenic: yes, in athymic mice

Products: amylase and other exocrine enzymes

Receptors expressed: insulin; glucocorticoid

Depositors: N.W. Jessop

The cells grow slowly, in clusters. They tend to pile up and appear refractile. Secretory activity is inducible by glucocorticoid stimulation, and is accompanied by extensive re-organization of the endoplasmic reticulum.

Features of The Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Specifically optimized to deliver nucleic acids into AR42J Cells
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Compatible with serum
• Suitable for Reverse Transfection
• Compatible with transfection in any plate formats

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on AR42J cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for AR42J cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this AR42J Cell Avalanche Transfection Reagent.

For Other Cells

AR42J Cell Avalanche® Transfection Reagent (rat pancreas tumor cell) can also be used on the following cells with high transfection efficiencies.

Capan-1 Cell

PANC-1 Cell

BxPC-3 Cell

AsPC-1 Cell

293 Cell

293T/17 Cell

SK-OV-3 Cell

U-2 OS cell

Saos-2 Cell

SK-N-SH Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Documents

Protocols

EZT-AR42-1 Protocol.

MSDS

EZT-AR42-1 MSDS

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