A549 Cell Avalanche® Transfection Reagent

A549 Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting A549 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for A549 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

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$714.00$1,264.00

 
Description

Cell to transfect:

Designations: A549

Cell Synonyms: : CCL-185, NCI60, NCI-60, human lung carcinoma cell

Depositors: M Lieber

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Organ: lung

Disease: carcinoma

Isolation: Isolation date: 1972

Applications: transfection host

Isoenzymes: G6PD, B

Age: 58 years

Gender: male

Ethnicity: Caucasian

This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male. Further studies by M. Lieber, et al. revealed that A549 cells could synthesize lecithin with a high percentage of desaturated fatty acids utilizing the cytidine diphosphocholine pathway. The cells are positive for keratin by immunoperoxidase staining.

A549 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

Features of The Transfection Reagent:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Unique formulation-maximize transfection performance in A549 cells.
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Compatible with serum
  • Compatible with transfection in any plate formats
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems

Data®

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on A549 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for A549 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this A549 Cell Avalanche Transfection Reagent.

For Other Cells

 

A549 Cell Avalanche® Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies.

NCI-H292 Cell

NCI-H460 Cell

NCI-H1299 Cell

NCI-H23 Cell

NCI-H1975 Cell

NCI-H441 Cell

Calu-3 Cell

NCI-H358 Cell

293 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype Adherent
Cell Type Epithelial Cell
Disease Cancer
Names starting from A
Primary/Cell Line Cell Line
Product Sizes 0.5 ml, 1.5 ml
Species Human
Tissue Sources Lung
Subcategories Cell Type/Cell Line Specific

Documents


Protocols

MSDS


Citations or Feedback

  • Siegers, J. Y., Ferreri, L., Eggink, D., Veldhuis Kroeze, E. J. B., Te Velthuis, A. J. W., van de Bildt, M., . . . van Riel, D. (2023). Evolution of highly pathogenic H5N1 influenza A virus in the central nervous system of ferrets. PLoS Pathog, 19(3), e1011214. doi:10.1371/journal.ppat.1011214

Testimonials and Citations

 
Description

Cell to transfect:

Designations: A549

Cell Synonyms: : CCL-185, NCI60, NCI-60, human lung carcinoma cell

Depositors: M Lieber

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Organ: lung

Disease: carcinoma

Isolation: Isolation date: 1972

Applications: transfection host

Isoenzymes: G6PD, B

Age: 58 years

Gender: male

Ethnicity: Caucasian

This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male. Further studies by M. Lieber, et al. revealed that A549 cells could synthesize lecithin with a high percentage of desaturated fatty acids utilizing the cytidine diphosphocholine pathway. The cells are positive for keratin by immunoperoxidase staining.

A549 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

Features of The Transfection Reagent:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Unique formulation-maximize transfection performance in A549 cells.
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Compatible with serum
  • Compatible with transfection in any plate formats
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems

Data®

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on A549 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for A549 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this A549 Cell Avalanche Transfection Reagent.

For Other Cells

 

A549 Cell Avalanche® Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies.

NCI-H292 Cell

NCI-H460 Cell

NCI-H1299 Cell

NCI-H23 Cell

NCI-H1975 Cell

NCI-H441 Cell

Calu-3 Cell

NCI-H358 Cell

293 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype Adherent
Cell Type Epithelial Cell
Disease Cancer
Names starting from A
Primary/Cell Line Cell Line
Product Sizes 0.5 ml, 1.5 ml
Species Human
Tissue Sources Lung
Subcategories Cell Type/Cell Line Specific

Documents


Protocols

MSDS


Citations or Feedback

  • Siegers, J. Y., Ferreri, L., Eggink, D., Veldhuis Kroeze, E. J. B., Te Velthuis, A. J. W., van de Bildt, M., . . . van Riel, D. (2023). Evolution of highly pathogenic H5N1 influenza A virus in the central nervous system of ferrets. PLoS Pathog, 19(3), e1011214. doi:10.1371/journal.ppat.1011214

Testimonials and Citations

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