Description
Cell to transfect:
Designations: HT-1080
Cell Synonyms: CCL-121, HT1080, human fibrosarcoma cell
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: connective tissue
Disease: fibrosarcoma
Isolation date: July, 1972
Applications: transfection host
Virus Susceptibility:Human poliovirus 1, RD-114 Feline, Feline leukemia virus, Vesicular stomatitis virus
Tumorigenic: Yes
Oncogene: ras +
Isoenzymes: G6PD, B
Age: 35 years
Gender: male
Ethnicity: Caucasian
The cells contain an activated N-ras oncogene.
Features of The Transfection Reagent:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Specifically optimized to deliver nucleic acids into HT-1080 cells
- Highest efficiency to ensure experimental success
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Compatible with serum
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on HT-1080 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for HT-1080 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this HT-1080 Cell Avalanche Transfection Reagent.
For Other Cells
HT-1080 Cell Avalanche® Transfection Reagent (human fibrosarcoma cell) can also be used on the following cells with high transfection efficiencies.
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
PC-3 Cell
AR42J Cell
Capan-1 Cell
PANC-1 Cell
BxPC-3 Cell
AsPC-1 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype |
Adherent |
| Cell Type |
Epithelial Cell |
| Disease |
Cancer |
| Names starting from |
H |
| Primary/Cell Line |
Cell Line |
| Product Sizes |
0.5 ml, 1.5 ml |
| Species |
Human |
| Tissue Sources |
Connective Tissue |
| Subcategories |
Cell Type/Cell Line Specific |
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