T98G Cell Avalanche® Transfection Reagent (human glioblastoma cell)

 
Description

Cell to transfect:

Designations: T98G

Cell Synonyms: T98-G, CRL-1690, human glioblastoma cell

Depositors: GH Stein

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: brain

Disease: glioblastoma multiforme

Applications: transfection host

Tumorigenic: No

Age: 61 years

Gender: male

Ethnicity: Caucasian

Comments: When deprived of serum or when crowded, the cells enter a viable G1 arrested state. The cells are anchorage independent.

Features of The Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Unique formulation-maximize transfection performance in T98G Cells.
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on T98G cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for T98G cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this T98G Cell Avalanche Transfection Reagent.

FIG. 2. T98G cells were transfected with GFP vector (pEGFP-N3) by using T98G Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells

T98G Cell Avalanche® Transfection Reagent (human glioblastoma cell) can also be used on the following cells with high transfection efficiencies.

MRC-5 Cell

IMR-90 Cell

COS-7 Cell

BHK-21 Cell

3197-3 Cell

NIH/3T3 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Documents

Protocols

EZT-T98G-1 Protocol.

MSDS

EZT-T98G-1 MSDS

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