EZT-U937-1 Protocol.
Description
Cell to transfect:
Designations: U-937
Cell Synonyms: : CRL-1593.2 , U937, U 937, human lymphoma cell
Morphology: monocyte
Source: histiocytic lymphoma
Culture Properties: suspension
Cellular Products: lysozyme; beta-2-microglobulin (beta 2 microglobulin); tumor necrosis factor (TNF) after stimulation with phorbol myristic acid (PMA)
Age: 37 years
Gender: male
Ethnicity: Caucasian
The U-937 cell line was derived by Sundstrom and Nilsson in 1974 from malignant cells obtained from the pleural effusion of a patient with histiocytic lymphoma.
Studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures,
phorbol esters, vitamin D3, gamma interferon, tumor necrosis factor (TNF) and, retinoic acid. The cells express the Fas antigen, and are sensitive to TNF and anti-Fas antibodies.
Features of The Transfection Reagent:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Specifically optimized to deliver nucleic acids into U-937 Cells
- Highest efficiency to ensure experimental success
- Extremely gentle to cells
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Animal-free
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on U-937 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for U-937 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this U-937 Cell Avalanche Transfection Reagent.
For Other Cells
U-937 Cell Avalanche® Transfection Reagent (human lymphoma cell) can also be used on the following cells with high transfection efficiencies.
293 Cell
293T/17 Cell
NIH/3T3 Cell
3T3-L1 Cell
BALB/3T3 clone A31
3T3-Swiss albino Cell
CT26.WT Cell
NCTC Clone 929 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype |
Suspension |
| Cell Type |
lymphocyte |
| Disease |
Cancer |
| Names starting from |
U |
| Primary/Cell Line |
Cell Line |
| Product Sizes |
0.5 ml, 1.5 ml |
| Species |
Human |
| Tissue Sources |
Lymphoma |
| Subcategories |
Cell Type/Cell Line Specific |
Documents
Protocols
MSDS
Citations or Feedback
