T84 Cell Avalanche® Transfection Reagent (human colon carcinoma cell)

 
Description

Cell to transfect:

Designations: T84

Cell Synonyms: CCL-248, colon carcinoma, cancer,® human colon carcinoma cell

Depositors: H Masui

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: colon

Disease: colorectal carcinoma

Derived from metastatic site: lung

Cellular Products: carcinoembryonic antigen (CEA), 600 ng/ml per 10 exp6 cells per 10 days; keratin

Applications: transfection host

Receptors: neurotransmitter, peptide hormone

Tumorigenic: Yes

Age: 72 year

Gender: male

Comments: The T84 cell line is a transplantable human carcinoma cell line derived from a lung metastasis of a colon carcinoma in a 72-year-old male. After 23 passages in athymic mice, the T84 cell line was established. These cells grow to confluence as monolayers and exhibit tight junctions and desmosomes between adjacent cells. They have receptors for many peptide hormones and neurotransmitters and maintain vectorial electrolyte transport. This line exhibits tight junctions, and desmosomes between adjacent cells. The cells are positive for keratin by immunoperoxidase staining.

Features of The Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Unique formulation-maximize transfection performance in T84 cells.
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Efficient for plasmid DNA/siRNA co-transfection
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on T84 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for T84 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this T84 Cell Avalanche Transfection Reagent.

For Other Cells

T84 Cell Avalanche® Transfection Reagent (human colon carcinoma cell) can also be used on the following cells with high transfection efficiencies.

HCT 116 Cell

DLD-1 Cell

HT-29 Cell

Caco-2 Cell

LS 174T Cell

LoVo Cell

SW480 Cell

SW620 Cell

293 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Documents

Protocols

EZT-T840-1 Protocol.

MSDS

EZT-T840-1 MSDS

Citations or Feedback

• Liu, D., Marie, J. C., Pelletier, A. L., Song, Z., Ben-Khemis, M., Boudiaf, K., . . . Dang, P. M. (2022). Protein Kinase CK2 Acts as a Molecular Brake to Control NADPH Oxidase 1 Activation and Colon Inflammation. Cell Mol Gastroenterol Hepatol, 13(4), 1073-1093. doi:10.1016/j.jcmgh.2022.01.003
• Makhezer, N., Ben Khemis, M., Liu, D., Khichane, Y., Marzaioli, V., Tlili, A., . . . Dang, P. M. (2019). NOX1-derived ROS drive the expression of Lipocalin-2 in colonic epithelial cells in inflammatory conditions. Mucosal Immunol, 12(1), 117-131. doi:10.1038/s41385-018-0086-4