SW480 Cell Avalanche® Transfection Reagent (human colon adenocarcinoma cell)

 
Description

Cell to transfect:

Designations: SW480 [SW-480]

Cell Synonyms: : SW-480, CCL-228, colon carcinoma, cancer, human colon adenocarcinoma cell

Depositors: A Leibovitz

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: colon

Tumor Stage: Dukes’ type B

Disease: colorectal adenocarcinoma

Cellular Products: carcinoembryonic antigen (CEA) 0.7 ng/10 exp6 cells/10 days; keratin; transforming growth factor beta

Applications: transfection host

Receptors: epidermal growth factor (EGF)

Virus Susceptibility: Human immunodeficiency virus 1

Tumorigenic: Yes

Antigen Expression: HLA A2, B8, B17; blood type A; Rh+

Age: 50 years

Gender: male

Ethnicity: Caucasian

Comments: SW480 was established from a primary adenocarcinoma of the colon. The line is negative for CSAp (CSAp-) and colon antigen 3, and is positive for keratin by immunoperoxidase staining. The cells express elevated levels of p53 protein. The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes. The cells have been reported to produce GM-CSF.

Features of The Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Unique formulation-maximize transfection performance in SW480 cells.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Efficient for plasmid DNA/siRNA co-transfection
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Compatible with serum
• Suitable for Reverse Transfection
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on T84 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for T84 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on SW480 cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as SW480 Cell Avalanche® Transfection Reagent.

For Other Cells

SW480 Cell Avalanche® Transfection Reagent (human colon adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies.

T84 Cell

HCT 116 Cell

DLD-1 Cell

HT-29 Cell

Caco-2 Cell

LS 174T Cell

LoVo Cell

SW620 Cell

293 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

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