RBL-2H3 Cell Avalanche® Transfection Reagent (rat leukemia cell)

 
Description

Cell to transfect:

Designations: RBL-2H3

Cell Synonyms: : CRL-2256, rat leukemia cell

Depositors: RP Siraganian

Growth Properties: adherent

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: fibroblast

Source: peripheral blood

Strain: Wistar

Disease: basophilic leukemia

Cell Type: basophil; chemically induced

Cellular Products: histamine

Applications: transfection host

Receptors: FcERI (Fc of IgE)

Comments: RBL-2H3 is a basophilic leukemia cell line isolated and cloned in 1978 from Wistar rat basophilic cells that were maintained as tumors.

These cells have high affinity IgE receptors. They can be activated to secrete histamine and other mediators by aggregation of these receptors or with calcium ionophores. They have been used extensively to study FcERI and the biochemical pathways for secretion in mast cells. They have also been used extensively for studies of different aspects of secretion in cells including the role of changes in intracellular calcium, the activation of phospholipases, protein kinases and small G proteins.

Features of The Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Unique formulation-maximize transfection performance in RBL-2H3 cells.
• Extremely gentle to cells
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Compatible with serum
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on RBL-2H3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for RBL-2H3 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this RBL-2H3 Cell Avalanche® Transfection Reagent.

For Other Cells

RBL-2H3 Cell Avalanche® Transfection Reagent (rat leukemia cell) can also be used on the following cells with high transfection efficiencies.

786-O Cell

Caki-1 Cell

MDCK Cell

Vero Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Documents

Protocols

MSDS

EZT-RBL2-1 MSDS

Citations or Feedback

• Liu, S., Sahid, M. N., Takemasa, E., Kiyoi, T., Kuno, M., Oshima, Y., & Maeyama, K. (2016). CRACM3 regulates the stability of non-excitable exocytotic vesicle fusion pores in a Ca(2+)-independent manner via molecular interaction with syntaxin4. Sci Rep, 6, 28133. doi:10.1038/srep28133
• Sahid, M. N. A., Liu, S., Mogi, M., & Maeyama, K. (2020). Tachykinin-1 receptor antagonism suppresses substance-P- and compound 48/80-induced mast cell activation from rat mast cells expressing functional mas-related GPCR B3. Inflamm Res, 69(3), 289-298. doi:10.1007/s00011-020-01319-z
• Suzuki, Y., Liu, S., Ogasawara, T., Sawasaki, T., Takasaki, Y., Yorozuya, T., & Mogi, M. (2020). A novel MRGPRX2-targeting antagonistic DNA aptamer inhibits histamine release and prevents mast cell-mediated anaphylaxis. Eur J Pharmacol, 878, 173104. doi:10.1016/j.ejphar.2020.173104