RAW 264.7 Cell Avalanche® Transfection Reagent (mouse macrophage tumor)

 
Description

Cell to transfect:

Designations: RAW 264.7

Cell Synonyms: TIB-71, RAW264® mouse macrophage tumor

Depositors: WC Raschke

Growth Properties: adherent

Organism: Mus musculus

Morphology: monocyte/macrophage

Strain: BALB/c

Tissue: ascites

Disease: Abelson murine leukemia virus-induced tumor

Cell Type: macrophage; Abelson murine leukemia virus transformed

Cellular Products: lysozyme

Applications: Biological response; transfection host

Antigen Expression: H-2d

Age: adult

Gender: male

Comments: This line was established from a tumor induced by Abelson murine leukemia virus. They are capable of antibody dependent lysis of sheep erythrocytes and tumor cell targets. LPS or PPD treatment for 2 days stimulates lysis of erythrocytes but not tumor cell targets.

Features of The Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Specifically optimized to deliver nucleic acids into RAW 264.7 cells
• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on RAW 264.7 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for RAW 264.7 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this RAW 264.7 Cell Avalanche Transfection Reagent.

For Other Cells

RAW 264.7 Cell Avalanche® Transfection Reagent (mouse macrophage tumor) can also be used on the following cells with high transfection efficiencies.

J774A.1 Cell

786-O Cell

Caki-1 Cell

MDCK Cell

Vero Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Documents

Protocols

EZT-RAW2-1 Protocol.

MSDS

EZT-RAW2-1 MSDS

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