EZT-RAMO-1 Protocol.
Description
Cell to transfect:
Designations: Ramos (RA 1)
Cell Synonyms: : RA1, CRL-1596, human lymphoma cell
Depositors: BC Giovanella
Isotype: IgM; lambda light chain
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Burkitt’s lymphoma (American)
Cell Type: B lymphocyte;
Cellular Products: immunoglobulin (surface and secreted) [1093] [22747].
Applications: transfection host
Receptors: interleukin 4 (IL-4); low affinity IgE (CD23) [23468]
Tumorigenic: Yes
Antigen Expression: CD23 + [23468]
Age: 3 years
Gender: male
Ethnicity: Caucasian
Comments: The cells are negative for Epstein-Barr virus. They have about 1500 IL-4 binding sites per cell.
The cells are reported to secrete IgM (lambda light chain).
Features of The Transfection Reagent:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Specifically optimized to deliver nucleic acids into Ramos cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Compatible with serum
- Suitable for Reverse Transfection
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on RAMOS cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for RAMOS cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this RAMOS Cell Avalanche® Transfection Reagent.
For Other Cells
Ramos Cell Avalanche® Transfection Reagent (human lymphoma cell) can also be used on the following cells with high transfection efficiencies.
293 Cell
293T/17 Cell
DU 145 Cell
22Rv1 Cell
VCaP Cell
Capan-1 Cell
PANC-1 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype |
Suspension |
| Cell Type |
lymphocyte |
| Disease |
Cancer |
| Names starting from |
R |
| Primary/Cell Line |
Cell Line |
| Product Sizes |
0.5 ml, 1.5 ml |
| Species |
Human |
| Tissue Sources |
Lymphoma |
| Subcategories |
Cell Type/Cell Line Specific |
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