EZT-RAJI-1 Protocol.
Description
Cell to transfect:
Designations: Raji
Cell Synonyms: : CCL-86, human lymphoma cell
Depositors: W Henle
Growth Properties: suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Burkitt’s lymphoma
Cell Type: B lymphocyte;
Isolation date: 1963
Applications: transfection host
Virus Resistance: The cells are partially resistant to poliovirus and vesicular stomatitis viruses.
Age: 11 years
Gender: male
Ethnicity: Black
Comments: The Raji line of lymphoblast-like cells was established by R.J.V. Pulvertaft in 1963 from a Burkitt’s lymphoma of the left maxilla of an 11-year-old Black male. EBNA positive.
Features of The Transfection Reagent:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Unique formulation-maximize transfection performance in Raji cells.
- Extremely gentle to cells
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Deliver single or multiple plasmids
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Compatible with serum
- Compatible with transfection in any plate formats
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on RAJI cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for RAJI cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this RAJI Cell Avalanche® Transfection Reagent.
For Other Cells
Raji Cell Avalanche® Transfection Reagent (human lymphoma cell) can also be used on the following cells with high transfection efficiencies.
Vero Cell
293 Cell
VCaP Cell
PC-3 Cell
AR42J Cell
Capan-1 Cell
PANC-1 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype |
Suspension |
| Cell Type |
lymphocyte |
| Disease |
Cancer |
| Names starting from |
R |
| Primary/Cell Line |
Cell Line |
| Product Sizes |
0.5 ml, 1.5 ml |
| Species |
Human |
| Tissue Sources |
Lymphoma |
| Subcategories |
Cell Type/Cell Line Specific |
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