PC-12 Cell Avalanche® Transfection Reagent (rat pheochromocytoma cell)

Name: PC-12 Cell Avalanche® Transfection Reagent (rat pheochromocytoma cell)
SKU: EZT-PC12-1
Availability: InStock

Transfection of suspension/adherent mixed cells, such as PC-12 cells, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments. However, electroporation usually leads to cell death, primarily when the electrical fields cause permanent permeabilization of the membrane and the consequent loss of cell homeostasis, in a process known as irreversible electroporation.

PC-12 Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting PC-12 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for PC-12 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

$714.00 – $1,264.00

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SKU: EZT-PC12-1 Categories: All Cell Lines, Cell Type/Cell Line-specific Transfection Reagent, For Cell Lines, P-T, Transfection Reagents Tags: adrenal gland, B Patterson, catecholamines, CRL-1721, dopamine, ngf, norepinephrine, PC-12, PC12, pheochromocytoma, rat pheochromocytoma cell

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$714.00 – $1,264.00

Description

Cell to transfect:

Designations: PC-12

Cell Synonyms: PC12, CRL-1721, pheochromocytoma, adrenal gland, rat pheochromocytoma cell

Organism: Rattus norvegicus (rat)

Tissue: pheochromocytoma; adrenal gland

Gender: male

Morphology: small irregularly shaped cells

Tumorigenic: yes, in New England Deaconess Hospital strain rats

Karyotype: 40 chromosomes; 38 autosomes plus XY

Products: catecholamines; dopamine; norepinephrine

Receptors expressed: nerve growth factor (NGF)

Growth Properties: floating clusters; few scattered lightly attached cells.

Depositor: B. Patterson

Comments: The PC-12 cell line was derived from a transplantable rat pheochromocytoma. The cells respond reversibly to NGF by induction of the neuronal phenotype when plated on Collagen IV coated culture flasks. The cells do not synthesize epinephrine.

Features of the The Transfection Reagent:

Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Specifically optimized to deliver nucleic acids into PC-12 cells
Highest efficiency to ensure experimental success
Extremely gentle to cells
0.5 ml is able to transfect about 1000 wells of 24-well plate
Deliver single or multiple plasmids
100% animal-free
Compatible with serum
Suitable for Reverse Transfection
Compatible with transfection in any plate formats
Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
Economical: High efficiency means less amount of nucleic acid & reagent is needed
Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on PC-12 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for PC-12 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this PC-12 Cell Avalanche Transfection Reagent.

For Other Cells

PC-12 Cell Avalanche® Transfection Reagent (rat pheochromocytoma cell) can also be used on the following cells with high transfection efficiencies.

786-O Cell

Caki-1 Cell

MDCK Cell

Vero Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight	0.5 lbs
Adherence Phenotype	Mixed
Cell Type	Others
Disease	Cancer
Names starting from	P
Primary/Cell Line	Cell Line
Product Sizes	0.5 ml, 1.5 ml
Species	Rat
Tissue Sources	Adrenal Gland
Subcategories	Cell Type/Cell Line Specific

Documents

Protocols

EZT-PC12-1 Protocol.

MSDS

EZT-PC12-1 MSDS

Citations or Feedback

Benicky, J., Sanda, M., Brnakova Kennedy, Z., & Goldman, R. (2019). N-Glycosylation is required for secretion of the precursor to brain- derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures. J Biol Chem, 294(45), 16816-16830. doi:10.1074/jbc.RA119.009989