PANC-1 Cell Avalanche® Transfection Reagent (human pancreas adenocarcinoma cell)

 
Description

Cell to transfect:

Designations: PANC-1

Cell Synonyms: CRL-1469, PANC1, human pancreas adenocarcinoma cell

Organism: Homo sapiens (human)

Tissue: pancreas; duct; epithelioid carcinoma

Age: 56 years

Gender: male

Ethnicity: caucasian

Morphology: epithelial

Growth properties: adherent

Depositors: M. Lieber

Growth is inhibited by 1 unit/ml L-asparaginase. The cells will grow in soft agar.

Features of The Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Specifically optimized to deliver nucleic acids into PANC-1 cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on Capan-1 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for Capan-1 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on PANC-1 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as PANC-1 Cell Avalanche Transfection Reagent.

For Other Cells

PANC-1 Cell Avalanche® Transfection Reagent (human pancreas adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies.

AR42J Cell

Capan-1 Cell

BxPC-3 Cell

AsPC-1 Cell

293 Cell

293T/17 Cell

SK-OV-3 Cell

U-2 OS cell

Saos-2 Cell

SK-N-SH Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Documents

Protocols

EZT-PANC-1 Protocol.

MSDS

EZT-PANC-1 MSDS

Citations or Feedback

• Tandon, M., Coudriet, G. M., Criscimanna, A., Socorro, M., Eliliwi, M., Singhi, A. D., . . . Esni, F. (2019). Prolactin Promotes Fibrosis and Pancreatic Cancer Progression. Cancer Res, 79(20), 5316-5327. doi:10.1158/0008-5472.Can-18-3064