NIH/3T3 Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on NIH/3T3 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for NIH/3T3 cells. No other transfection reagents can match the efficiency, convenience, and gentleness of NIH/3T3 Cell Avalanche® Transfection Reagent for transfection on NIH/3T3 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
Comments: The NIH/3T3 is highly sensitive to sarcoma virus focus formation and leukemia virus propagation and has proven to be very useful in DNA transfection studies [PubMed ID: 222457].
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Unique formulation-maximize transfection performance in NIH/3T3 cells.
Highest efficiency to ensure experimental success
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
0.5 ml is able to transfect about 1000 wells of 24-well plate
Animal-free
Compatible with serum
Suitable for Reverse Transfection
Compatible with transfection in any plate formats
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NIH/3T3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NIH/3T3 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this NIH/3T3 Cell Avalanche Transfection Reagent.
FIG. 2. NIH/3T3 cells were transfected with GFP vector (pEGFP-N3) by using NIH/3T3 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
NIH/3T3 Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
3197-3 Cell
3T3-L1 Cell
BALB/3T3 clone A31
3T3-Swiss albino Cell
786-O Cell
Caki-1 Cell
Detroit 551 Cell
MG-63 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Comments: The NIH/3T3 is highly sensitive to sarcoma virus focus formation and leukemia virus propagation and has proven to be very useful in DNA transfection studies [PubMed ID: 222457].
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Unique formulation-maximize transfection performance in NIH/3T3 cells.
Highest efficiency to ensure experimental success
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
0.5 ml is able to transfect about 1000 wells of 24-well plate
Animal-free
Compatible with serum
Suitable for Reverse Transfection
Compatible with transfection in any plate formats
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NIH/3T3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NIH/3T3 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this NIH/3T3 Cell Avalanche Transfection Reagent.
FIG. 2. NIH/3T3 cells were transfected with GFP vector (pEGFP-N3) by using NIH/3T3 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
NIH/3T3 Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
3197-3 Cell
3T3-L1 Cell
BALB/3T3 clone A31
3T3-Swiss albino Cell
786-O Cell
Caki-1 Cell
Detroit 551 Cell
MG-63 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
EZT-NIH3-1 Protocol.
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NIH/3T3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NIH/3T3 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this NIH/3T3 Cell Avalanche Transfection Reagent.
