EZT-NEUR-1 Protocol.
Description
Cell to transfect:
Designations: Neuro-2a
Cell Synonyms: CCL-131, N2a, mouse neuroblastoma cell
Organism: Mus musculus (mouse)
Strain: A
Genotype: albino
Tissue: brain; neuroblast; neuroblastoma
Morphology: neuronal and amoeboid stem cells
Growth properties: adherent
Antigen Expression: H-2a
Cellular products: tubulin; acetylcholinesterase
Depositor: R.J. Klebe
Neuro-2a was established by R.J. Klebe and F.H. Ruddle from a spontaneous tumor of a strain A albino mouse. Neuro-2a cells produce large quantities of microtubular protein which is believed to play a role in a contractile system which is responsible for axoplasmic flow in nerve cells. The cell line has been used for studies on the mechanism of vinblastine precipitation of microtubular protein, the kinetics of GTP binding to isolated protein, the turnover of microtubules in vivo, and the synthesis and assembly of microtubular protein. The World Organization for Animal Health (OIE) uses the cells for routine diagnosis of rabies.
Features of The Transfection Reagent:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Specifically optimized to deliver nucleic acids into Neuro-2a cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NEURO-2A cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NEURO-2A cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this NEURO-2A Cell Avalanche Transfection Reagent.
For Other Cells
Neuro-2a Cell Avalanche® Transfection Reagent (mouse neuroblastoma cell) can also be used on the following cells with high transfection efficiencies.
IMR-32 Cell
T98G Cell
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype |
Adherent |
| Cell Type |
Neural Cell |
| Disease |
Cancer |
| Names starting from |
N |
| Primary/Cell Line |
Cell Line |
| Product Sizes |
0.5 ml, 1.5 ml |
| Species |
Mouse |
| Tissue Sources |
Neuroblastoma |
| Subcategories |
Cell Type/Cell Line Specific |
Documents
Protocols
MSDS
Citations or Feedback
- Hall, B. E., Prochazkova, M., Sapio, M. R., Minetos, P., Kurochkina, N., Binukumar, B. K., . . . Kulkarni, A. B. (2018). Phosphorylation of the Transient Receptor Potential Ankyrin 1 by Cyclin- dependent Kinase 5 affects Chemo-nociception. Sci Rep, 8(1), 1177. doi:10.1038/s41598-018-19532-6
