NCI-N87 Cell Avalanche® Transfection Reagent (human gastric carcinoma cell)

NCI-N87 Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting NCI-N87 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for NCI-N87 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

$714.00$1,264.00

& Free Shipping

$714.00$1,264.00

 
Description

Cell to transfect:

Designations: NCI-N87

Cell Synonyms: CRL-5822, N87, human gastric carcinoma cell

Depositors: J Park

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: stomach

Disease: gastric carcinoma

Derived from metastatic site: liver

Isolation date: 1976

Receptors: acetylcholine, muscarinic, expressed [23078]

Tumorigenic: Yes

Oncogene: myc +; erb B2 +

Cytogenetic Analysis: near diploid; DM were present in 64% of cells examined

Gender: male

Comments: NCI-N87 cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72, and are L-dopa decarboxylase (DDC) negative. They were minimally positive for vasoactive intestinal peptide (VIP) receptors and lacked gastrin receptors. They were found to express receptors for muscarinic cholinergic agents.

Features of The Transfection Reagent:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Unique formulation-maximize transfection performance in NCI-N87 cells.
  • Extremely gentle to cells
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Compatible with serum
  • Suitable for Reverse Transfection
  • Compatible with transfection in any plate formats
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NCI-N87 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NCI-N87 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this NCI-N87 Cell Avalanche Transfection Reagent.

For Other Cells

NCI-N87 Cell Avalanche® Transfection Reagent (human gastric carcinoma cell) can also be used on the following cells with high transfection efficiencies.

AGS Cell

Caki-1 Cell

MDCK Cell

Vero Cell

293 Cell

PC-3 Cell

AR42J Cell

Capan-1 Cell

PANC-1 Cell

BxPC-3 Cell

AsPC-1 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Adherent

Cell Type

Epithelial Cell

Disease

Cancer

Names starting from

N

Primary/Cell Line

Cell Line

Product Sizes

0.5 ml, 1.5 ml

Species

Human

Tissue Sources

Stomach

Subcategories

Cell Type/Cell Line Specific

Documents



Protocols


MSDS



Citations or Feedback

Testimonials and Citations

 
Description

Cell to transfect:

Designations: NCI-N87

Cell Synonyms: CRL-5822, N87, human gastric carcinoma cell

Depositors: J Park

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: stomach

Disease: gastric carcinoma

Derived from metastatic site: liver

Isolation date: 1976

Receptors: acetylcholine, muscarinic, expressed [23078]

Tumorigenic: Yes

Oncogene: myc +; erb B2 +

Cytogenetic Analysis: near diploid; DM were present in 64% of cells examined

Gender: male

Comments: NCI-N87 cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72, and are L-dopa decarboxylase (DDC) negative. They were minimally positive for vasoactive intestinal peptide (VIP) receptors and lacked gastrin receptors. They were found to express receptors for muscarinic cholinergic agents.

Features of The Transfection Reagent:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Unique formulation-maximize transfection performance in NCI-N87 cells.
  • Extremely gentle to cells
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Compatible with serum
  • Suitable for Reverse Transfection
  • Compatible with transfection in any plate formats
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on NCI-N87 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for NCI-N87 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this NCI-N87 Cell Avalanche Transfection Reagent.

For Other Cells

NCI-N87 Cell Avalanche® Transfection Reagent (human gastric carcinoma cell) can also be used on the following cells with high transfection efficiencies.

AGS Cell

Caki-1 Cell

MDCK Cell

Vero Cell

293 Cell

PC-3 Cell

AR42J Cell

Capan-1 Cell

PANC-1 Cell

BxPC-3 Cell

AsPC-1 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Adherent

Cell Type

Epithelial Cell

Disease

Cancer

Names starting from

N

Primary/Cell Line

Cell Line

Product Sizes

0.5 ml, 1.5 ml

Species

Human

Tissue Sources

Stomach

Subcategories

Cell Type/Cell Line Specific

Documents



Protocols


MSDS



Citations or Feedback

Testimonials and Citations

Shopping Cart
Scroll to Top