NCI-H460 Cell Avalanche® Transfection Reagent (human lung carcinoma cell)

 
Description

Cell to transfect:

Designations: NCI-H460

Cell Synonyms: HTB-177, H460, human lung carcinoma cell

Depositors: AF Gazdar, JD Minna

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Organ: lung

Disease: carcinoma; large cell lung cancer

Derived from metastatic site: pleural effusion

Isolation: Isolation date: 1982

Tumorigenic: Yes

Gender: male

The NCI-H460 cell line was derived by A.F. Gazdar and associates in 1982 from the pleural fluid of a patient with large cell cancer of the lung. The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities. The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein.

Features of The Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Specifically optimized to deliver nucleic acids into NCI-H460 Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Compatible with serum

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on A549 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for A549 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on NCI-H460 cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as NCI-H460 Cell Avalanche Transfection Reagent.

For Other Cells

NCI-H460 Cell Avalanche® Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies.

A549 Cell, NCI-H292 Cell, NCI-H1299 Cell, NCI-H23 Cell, NCI-H1975 Cell, NCI-H441 Cell, Calu-3 Cell, NCI-H358 Cell, 293 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc.

For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

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