NCI-H23 Cell Avalanche® Transfection Reagent (human lung carcinoma cell)

 
Description

Cell to transfect:

Designations: NCI-H23

Cell Synonyms: CRL-5800, NCI60, NCI-60, human lung carcinoma cell

Depositors: AF Gazdar, JD Minna

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Organ: lung

Disease: adenocarcinoma; non-small cell lung cancer

Oncogene: myc +; src +; abl +; erb +; ras +; sis –

Age: 51 years

Gender: male

Ethnicity: Black

NCI-23 was derived from a lung cancer obtained from a patient prior to therapy. The line exhibits a high degree of c-myc DNA amplification (20-fold) but no detectable amplification of c-myc RNA. The cells stain positive for keratins 5+8 and 18 and vimentin but are negative for neurofilament.

NIH-H23 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

Features of The Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Unique formulation-maximize transfection performance in NCI-H23 cells.
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Compatible with serum
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on A549 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for A549 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on NCI-H23 cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as NCI-H23 Cell Avalanche Transfection Reagent.

For Other Cells

NCI-H23 Cell Avalanche® Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies.

A549 Cell

NCI-H292 Cell

NCI-H460 Cell

NCI-H1299 Cell

NCI-H1975 Cell

NCI-H441 Cell

Calu-3 Cell

NCI-H358 Cell

293 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Documents

Citations or Feedback