MG-63 Cell Avalanche® Transfection Reagent (human osteosarcoma cell)

MG-63 Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on MG-63 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for MG-63 cells. No other transfection reagents can match the efficiency, convenience, and gentleness of MG-63 Cell Avalanche® Transfection Reagent for transfection on MG-63 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

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$714.00$1,264.00

 
Description

Cell to transfect:

Designations: MG-63

Cell Synonyms: CRL-1427, MG63, osteosarcoma, human osteosarcoma cell

Organism: Homo sapiens (human)

Tissue: osteosarcoma; bone

Age: 14 years

Gender: male

Ethnicity: caucasian

Morphology: fibroblast

Growth properties: adherent

Receptors expressed: transforming growth factor beta (TGF-beta) Rl and RII

Depositors: A. Billiau

High levels of interferon production can be induced using polyinosinic – polycytidylic acid, cycloheximide and actinomycinD. This is a hypotriploid human cell line. The modal chromosome number was 66 occurring in 44% of cells. The rate of cells with higher ploidies was 2.0%. Eighteen to 19 marker chromosomes were common to all cells.

Features of The Transfection Reagent:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Specifically optimized to deliver nucleic acids into MG-63 cells
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Compatible with serum
  • Suitable for Reverse Transfection
  • Compatible with transfection in any plate formats
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on MG-63 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for MG-63 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this MG-63 Cell Avalanche Transfection Reagent.

FIG. 2. MG-63 cells were transfected with GFP vector (pEGFP-N3) by using MG-63 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells

MG-63 Cell Avalanche® Transfection Reagent (human osteosarcoma cell) can also be used on the following cells with high transfection efficiencies.

MDCK Cell

Vero Cell

293 Cell

WI-38 Cell

MRC-5 Cell

IMR-90 Cell

COS-7 Cell

BHK-21 Cell

3197-3 Cell

NIH/3T3 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Adherent

Cell Type

Fibroblast

Disease

Cancer

Names starting from

M

Primary/Cell Line

Cell Line

Product Sizes

0.5 ml, 1.5 ml

Species

Human

Tissue Sources

Bone

Subcategories

Cell Type/Cell Line Specific

Documents



Protocols


MSDS



Citations or Feedback

Testimonials and Citations

 
Description

Cell to transfect:

Designations: MG-63

Cell Synonyms: CRL-1427, MG63, osteosarcoma, human osteosarcoma cell

Organism: Homo sapiens (human)

Tissue: osteosarcoma; bone

Age: 14 years

Gender: male

Ethnicity: caucasian

Morphology: fibroblast

Growth properties: adherent

Receptors expressed: transforming growth factor beta (TGF-beta) Rl and RII

Depositors: A. Billiau

High levels of interferon production can be induced using polyinosinic – polycytidylic acid, cycloheximide and actinomycinD. This is a hypotriploid human cell line. The modal chromosome number was 66 occurring in 44% of cells. The rate of cells with higher ploidies was 2.0%. Eighteen to 19 marker chromosomes were common to all cells.

Features of The Transfection Reagent:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Specifically optimized to deliver nucleic acids into MG-63 cells
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Compatible with serum
  • Suitable for Reverse Transfection
  • Compatible with transfection in any plate formats
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on MG-63 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for MG-63 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this MG-63 Cell Avalanche Transfection Reagent.

FIG. 2. MG-63 cells were transfected with GFP vector (pEGFP-N3) by using MG-63 Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells

MG-63 Cell Avalanche® Transfection Reagent (human osteosarcoma cell) can also be used on the following cells with high transfection efficiencies.

MDCK Cell

Vero Cell

293 Cell

WI-38 Cell

MRC-5 Cell

IMR-90 Cell

COS-7 Cell

BHK-21 Cell

3197-3 Cell

NIH/3T3 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Adherent

Cell Type

Fibroblast

Disease

Cancer

Names starting from

M

Primary/Cell Line

Cell Line

Product Sizes

0.5 ml, 1.5 ml

Species

Human

Tissue Sources

Bone

Subcategories

Cell Type/Cell Line Specific

Documents



Protocols


MSDS



Citations or Feedback

Testimonials and Citations

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