MDCK Cell Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on MDCK cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for MDCK cells. No other transfection reagents can match the efficiency, convenience, and gentleness of MDCK Cell Avalanche® Transfection Reagent for transfection on MDCK cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
Virus Susceptibility: Human Coxsackievirus B, Human Coxsackievirus B, Human Coxsackievirus B, Human poliovirus.
Age: adult
Gender: female
Comments: The MDCK cell line was derived from a kidney of an apparently normal adult female cocker spaniel, September, 1958, by S.H. Madin and N.B. Darby. The cells are positive for keratin by immunoperoxidase staining. MDCK cells have been used to study processing of beta amyloid precursor protein and sorting of its proteolytic products.
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Unique formulation-maximize transfection performance in MDCK Cells.
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
0.5 ml is able to transfect about 1000 wells of 24-well plate
Suitable for Reverse Transfection
Compatible with transfection in any plate formats
Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
Economical: High efficiency means less amount of nucleic acid & reagent is needed
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on MDCK Cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for MDCK Cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this MDCK Cell Avalanche Transfection Reagent.
FIG. 2. MDCK cells were transfected with GFP vector (pEGFP-N3) by using MDCK Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells®
MDCK Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Kajiwara, K., Yamano, S., Aoki, K., Okuzaki, D., Matsumoto, K., & Okada, M. (2021). CDCP1 promotes compensatory renal growth by integrating Src and Met signaling. Life Sci Alliance, 4(4). doi:10.26508/lsa.202000832
Tanaka, K., Ito, Y., Kajiwara, K., Nada, S., & Okada, M. (2020). Ubiquitination of Src promotes its secretion via small extracellular vesicles. Biochem Biophys Res Commun. doi:10.1016/j.bbrc.2020.02.057
Trease, A. J., Capuccino, J. M. V., Contreras, J., Harris, A. L., & Sorgen, P. L. (2017). Intramolecular signaling in a cardiac connexin: Role of cytoplasmic domain dimerization. J Mol Cell Cardiol, 111, 69-80. doi:10.1016/j.yjmcc.2017.07.010
Virus Susceptibility: Human Coxsackievirus B, Human Coxsackievirus B, Human Coxsackievirus B, Human poliovirus.
Age: adult
Gender: female
Comments: The MDCK cell line was derived from a kidney of an apparently normal adult female cocker spaniel, September, 1958, by S.H. Madin and N.B. Darby. The cells are positive for keratin by immunoperoxidase staining. MDCK cells have been used to study processing of beta amyloid precursor protein and sorting of its proteolytic products.
Features of The Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Unique formulation-maximize transfection performance in MDCK Cells.
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
0.5 ml is able to transfect about 1000 wells of 24-well plate
Suitable for Reverse Transfection
Compatible with transfection in any plate formats
Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
Economical: High efficiency means less amount of nucleic acid & reagent is needed
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on MDCK Cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for MDCK Cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this MDCK Cell Avalanche Transfection Reagent.
FIG. 2. MDCK cells were transfected with GFP vector (pEGFP-N3) by using MDCK Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells®
MDCK Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Kajiwara, K., Yamano, S., Aoki, K., Okuzaki, D., Matsumoto, K., & Okada, M. (2021). CDCP1 promotes compensatory renal growth by integrating Src and Met signaling. Life Sci Alliance, 4(4). doi:10.26508/lsa.202000832
Tanaka, K., Ito, Y., Kajiwara, K., Nada, S., & Okada, M. (2020). Ubiquitination of Src promotes its secretion via small extracellular vesicles. Biochem Biophys Res Commun. doi:10.1016/j.bbrc.2020.02.057
Trease, A. J., Capuccino, J. M. V., Contreras, J., Harris, A. L., & Sorgen, P. L. (2017). Intramolecular signaling in a cardiac connexin: Role of cytoplasmic domain dimerization. J Mol Cell Cardiol, 111, 69-80. doi:10.1016/j.yjmcc.2017.07.010
EZT-MDCK-1 Protocol.
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on MDCK Cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for MDCK Cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this MDCK Cell Avalanche Transfection Reagent.
