MDA-MB-468 Cell Avalanche® Transfection Reagent (human breast carcinoma cell)

 
Description

Cell to transfect:

Designations: MDA-MB-468

Cell Synonyms: : HTB-132, human breast carcinoma cell

Depositors: R Cailleau

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: mammary gland; breast

Disease: adenocarcinoma

Isolation date: 1977

Applications: transfection host

Receptors: epidermal growth factor (EGF), transforming growth factor alpha (TGF alpha)

Tumorigenic: Yes

Antigen Expression: Blood Type AB; HLA Aw23, Aw30, B27, Bw35, Cw2, Cw4 (patient)

Age: 51 years

Gender: female

Ethnicity: Black

Comments: The MDA-MB-468 cell line was isolated in 1977 by R. Cailleau, et al., from a pleural effusion of a 51-year-old Black female patient with metastatic adenocarcinoma of the breast. Although the tissue donor was heterozygous for the G6PD alleles, the cell line consistently showed only the G6PD A phenotype. There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution. EGF receptor is present at 1 X 10(6) per cell.

Features of the Transfection Reagent:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Specifically optimized to deliver nucleic acids into MDA-MB-468 cells
• Extremely gentle to cells
• 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Efficient for plasmid DNA/siRNA co-transfection
• Compatible with serum
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on MDA-MB-453 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for MDA-MB-453 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on MDA-MB-468 cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as MDA-MB-468 Cell Avalanche® Transfection Reagent.

FIG. 2. MDA-MB-468 cells were transfected with GFP vector (pEGFP-N3) by using MDA-MB-468 Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.

For Other Cells

MDA-MB-468 Cell Avalanche® Transfection Reagent (human breast carcinoma cell) can also be used on the following cells with high transfection efficiencies.

4T1 Cell

ZR-75-1 Cell

BT-20 Cell

MDA-MB-453 Cell

HCC1937 Cell

SK-BR-3 Cell

MDA-MB-231 Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

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