Malme-3M Cell Avalanche® Transfection Reagent (human melanoma cell)

Transfection of suspension/adherent mixed cells, such as Malme-3M cell, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments. However, electroporation usually leads to cell death, primarily when the electrical fields cause permanent permeabilization of the membrane and the consequent loss of cell homeostasis, in a process known as irreversible electroporation.

Malme-3M Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Malme-3M cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Malme-3M cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

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$714.00$1,264.00

Description

Cell to transfect:

Cell Line Name: Malme-3M (ATCC® HTB-64®)

Cell Synonyms: NCI60, NCI-60, HTB-64, human melanoma cell

Organism: Homo sapiens, human

Tissue Derived from metastatic site: lung

Cell Type: fibroblast

Morphology: mixed

Culture Properties: mixed adherent-suspension

Biosafety Level; 1

Disease: Malignant melanoma

Age: 43 years

Gender: male

Ethnicity: Caucasian

Applications: This melanoma cell line was isolated from the same patient as Malme-3 (ATCC HTB-102), a normal skin fibroblast. The two cell lines provide tumor and normal counterparts for comparative in vitro melanoma studies. This is one of an extensive series of human tumor lines isolated and characterized by J. Fogh.

Markers: HLA A2, Aw30, B13, B40(+/-), DRw7

Tumorigenic: Yes

Malme-3M is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent Features:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Unique formulation-maximize transfection performance in Malme-3M cells
  • Extremely gentle to cells
  • Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Free of animal-derived components
  • Active in serum-containing medium
  • Compatible with transfection in any plate format
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on Malme-3M cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for Malme-3M cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Malme-3M Cell Avalanche® Transfection Reagent.

For Other Cells

Malme-3M Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.

LOX-IMVI Cell

M14 Cell

SK-MEL-2 Cell

SK-MEL-5 Cell

UACC-257 Cell

UACC-62 Cell

Vero Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Mixed

Cell Type

Fibroblast

Disease

Cancer

Names starting from

M

Primary/Cell Line

Cell Line

Product Sizes

0.5 ml, 1.5 ml

Species

Human

Subcategories

Cell Type/Cell Line Specific

Tissue Sources

Melanoma

Documents

Protocol:

MSDS:

New Doc:

Citations or Feedback

Testimonials and Citations

Description

Cell to transfect:

Cell Line Name: Malme-3M (ATCC® HTB-64®)

Cell Synonyms: NCI60, NCI-60, HTB-64, human melanoma cell

Organism: Homo sapiens, human

Tissue Derived from metastatic site: lung

Cell Type: fibroblast

Morphology: mixed

Culture Properties: mixed adherent-suspension

Biosafety Level; 1

Disease: Malignant melanoma

Age: 43 years

Gender: male

Ethnicity: Caucasian

Applications: This melanoma cell line was isolated from the same patient as Malme-3 (ATCC HTB-102), a normal skin fibroblast. The two cell lines provide tumor and normal counterparts for comparative in vitro melanoma studies. This is one of an extensive series of human tumor lines isolated and characterized by J. Fogh.

Markers: HLA A2, Aw30, B13, B40(+/-), DRw7

Tumorigenic: Yes

Malme-3M is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent Features:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Unique formulation-maximize transfection performance in Malme-3M cells
  • Extremely gentle to cells
  • Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Free of animal-derived components
  • Active in serum-containing medium
  • Compatible with transfection in any plate format
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on Malme-3M cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for Malme-3M cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Malme-3M Cell Avalanche® Transfection Reagent.

For Other Cells

Malme-3M Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.

LOX-IMVI Cell

M14 Cell

SK-MEL-2 Cell

SK-MEL-5 Cell

UACC-257 Cell

UACC-62 Cell

Vero Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Mixed

Cell Type

Fibroblast

Disease

Cancer

Names starting from

M

Primary/Cell Line

Cell Line

Product Sizes

0.5 ml, 1.5 ml

Species

Human

Subcategories

Cell Type/Cell Line Specific

Tissue Sources

Melanoma

Documents

Protocol:

MSDS:

New Doc:

Citations or Feedback

Testimonials and Citations

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