M14 (MDA-MB-435) Cell Avalanche® Transfection Reagent

 
Description

Cells to transfect:

Cell Line Name: M14

Cell Synonyms: NCI60, NCI-60, MDA-MB-435

Tissue Type: Skin

Histology: Melanotic melanoma; Malignant melanoma; non epithelial

p53 Status: 0

ECAD Methylation; 35

MDR Function: 14

Institution: John Wayne Cancer clinic, UCLA School of Medicine

Contributor: Kern, DH

Reference: Cancer Res. 48:578-582, 1988

Comments: MDA-MB-435 was the result of contamination of M14 cells. M14 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

Transfection reagent features:

• Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
• Specifically optimized to deliver nucleic acids into M14 cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Active in serum-containing medium
• Suitable for Reverse Transfection
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on M14 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for M14 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this M14 Cell Avalanche® Transfection Reagent.

FIG. 2. M14 cells were transfected with GFP vector (pEGFP-N3) by using M14 Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells

M14 (MDA-MB-435) Cell Avalanche® Transfection Reagent (human melanoma cell) can also be used on the following cells with high transfection efficiencies.

LOX-IMVI Cell

SK-MEL-2 Cell

SK-MEL-5 Cell

UACC-257 Cell

UACC-62 Cell

MDCK Cell

Vero Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Documents

Protocols

EZT-M140-1 Protocol.

MSDS

EZT-M140-1 MSDS

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