Description
Cell to transfect:
Designations: LS 174T
Cell Synonyms: CL-188, colon carcinoma, cancer, human colon adenocarcinoma cell
Depositors: Northwestern University
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: colon
Tumor Stage: Dukes’ type B
Disease: colorectal adenocarcinoma
Cellular Products: carcinoembryonic antigen (CEA), interleukin 10 (IL-10), interleukin 6 (IL-6), mucin
Applications: Electron microscopic studies revealed abundant microvilli and intracytoplasmic mucin vacuoles
Tumorigenic: Yes
Age: 58 years
Gender: female
Ethnicity: Caucasian
Comments: LS 174T is a variant of LS 180 (ATCC CL-187) that has been maintained by using trypsin in the subculture protocol. It is more easily subcultivated than that parent line. Electron microscopic studies revealed abundant microvilli and intracytoplasmic mucin vacuoles. LS 174T cells stain positively for cytokeratins. The line is positive for expression of c-myc, N-myc, H-ras, N-ras, Myb, and fos oncogenes.
Features of The Transfection Reagent:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Specifically optimized to deliver nucleic acids into LS 174T cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Compatible with transfection in any plate formats
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on T84 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for T84 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on LS 174T cells by transfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as LS 174T Cell Avalanche Transfection® Reagent.
For Other Cells
LS 174T Cell Avalanche® Transfection Reagent (human colon adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies.
T84 Cell
HCT 116 Cell
DLD-1 Cell
HT-29 Cell
Caco-2 Cell
LoVo Cell
SW480 Cell
SW620 Cell
293 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
Weight | 0.5 lbs |
---|---|
Adherence Phenotype |
Adherent |
Cell Type |
Epithelial Cell |
Disease |
Cancer |
Names starting from |
L |
Primary/Cell Line |
Cell Line |
Product Sizes |
0.5 ml, 1.5 ml |
Species |
Human |
Tissue Sources |
Colon |
Subcategories |
Cell Type/Cell Line Specific |
Documents
Protocols
MSDS
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