Transfection of cells that form loosely attached clusters, such as LNCaP cells, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments. However, electroporation usually leads to cell death, primarily when the electrical fields cause permanent permeabilization of the membrane and the consequent loss of cell homeostasis, in a process known as irreversible electroporation.
LNCaP Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting LNCaP cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for LNCaP cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
Cell Synonyms: LNCaP Clone FGC, CRL-1740, human prostate carcinoma cell
Organism: Homo sapiens (human)
Tissue: prostate carcinoma; metastatic site: left supraclavicular lymph node
Age: 50 years
Gender: male
Ethnicity: caucasian
Morphology: epithelial
Growth Properties: clusters; lightly adherent
Doubling time: about 34 hours
Tumorigenic: yes, in nude mice
Products: human prostatic acid phosphatase; prostate specific antigen (PSA)
Receptors expressed: androgen; estrogen
Depositors: J.S. Horoszewicz
This line was isolated in 1977 by Horoszewicz et al from a needle aspiration biopsy of the left supraclavicular lymph node of a 50-year-old Caucasian male with confirmed diagnosis of metastatic prostate carcinoma. These cells are responsive to 5-alpha-dihydrotestosterone (growth modulation and acid phosphatase production).
Features of the Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Specifically optimized to deliver nucleic acids into LNCaP cells
Highest efficiency to ensure experimental success
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
0.5 ml is able to transfect about 1000 wells of 24-well plate
Deliver single or multiple plasmids
Compatible with serum
Suitable for Reverse Transfection
Compatible with transfection in any plate formats
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on LNCAP cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for LNCAP cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this LNCAP Cell Avalanche Transfection Reagent.
FIG. 2. LNCaP cells were transfected with GFP vector (pEGFP-N3) by using LNCaP Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
LNCaP Cell Avalanche® Transfection Reagent (human prostate carcinoma cell) can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Cell Synonyms: LNCaP Clone FGC, CRL-1740, human prostate carcinoma cell
Organism: Homo sapiens (human)
Tissue: prostate carcinoma; metastatic site: left supraclavicular lymph node
Age: 50 years
Gender: male
Ethnicity: caucasian
Morphology: epithelial
Growth Properties: clusters; lightly adherent
Doubling time: about 34 hours
Tumorigenic: yes, in nude mice
Products: human prostatic acid phosphatase; prostate specific antigen (PSA)
Receptors expressed: androgen; estrogen
Depositors: J.S. Horoszewicz
This line was isolated in 1977 by Horoszewicz et al from a needle aspiration biopsy of the left supraclavicular lymph node of a 50-year-old Caucasian male with confirmed diagnosis of metastatic prostate carcinoma. These cells are responsive to 5-alpha-dihydrotestosterone (growth modulation and acid phosphatase production).
Features of the Transfection Reagent:
Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Specifically optimized to deliver nucleic acids into LNCaP cells
Highest efficiency to ensure experimental success
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
0.5 ml is able to transfect about 1000 wells of 24-well plate
Deliver single or multiple plasmids
Compatible with serum
Suitable for Reverse Transfection
Compatible with transfection in any plate formats
Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on LNCAP cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for LNCAP cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this LNCAP Cell Avalanche Transfection Reagent.
FIG. 2. LNCaP cells were transfected with GFP vector (pEGFP-N3) by using LNCaP Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
LNCaP Cell Avalanche® Transfection Reagent (human prostate carcinoma cell) can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
EZT-LNCP-1 Protocol.
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on LNCAP cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for LNCAP cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this LNCAP Cell Avalanche Transfection Reagent.
