EZT-L600-1 Protocol.
Description
Cell to transfect:
Designations: L6
Cell Synonyms: : CRL-1458
Organism: Rattus norvegicus (rat)
Tissue: skeletal muscle; myoblast
Morphology: myoblast
Growth properties: adherent
Products: myosin
Depositors: D. Schubert
The L6 myogenic line was isolated originally by Yaffe from primary cultures of rat thigh muscle maintained for the first two passages in the presence of methyl cholanthrene.
L6 cells fuse in culture to form multinucleated myotubes and striated fibers. The myoblastic component of this line will be depleted rapidly if the cells are allowed to become confluent.
The Transfection Reagent Features:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Specifically optimized to deliver nucleic acids into L6 cells
- Highest efficiency to ensure experimental success
- Extremely gentle to cells
- Broad spectrum on nucleic acids: All Avalanche® transfection reagents share the same feature: being able to transfect all type of nucleic acids, such as plasmid DNA (large or small), mRNA, siRNA, micro RNA, and other oligo nucleic acids, which is the best for co-transfection.
- Reliable performance for high-throughput applications
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Developed and manufactured by EZ Biosystems
Data：
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on L6 Cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for L6 Cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this L6 Cell Avalanche Transfection Reagent.
For Other Cells：
L6 Cell Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
H9c2(2-1)
C2C12 Cell
786-O Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.1 lbs |
|---|---|
| Dimensions | 0.5 × 0.2 × 0.5 in |
| Adherence Phenotype | Adherent |
| Cell Type | Myoblast |
| Disease | Healthy |
| Names starting from | L |
| Primary/Cell Line | Cell Line |
| Product Sizes | 0.5 ml, 1.5 ml |
| Species | Rat |
| Subcategories | Cell Type/Cell Line Specific |
| Tissue Sources | Skeletal Muscle |
Documents
Protocols
MSDS
Citations or Feedback
- Merz, K. E., Hwang, J., Zhou, C., Veluthakal, R., McCown, E. M., Hamilton, A., . . . Thurmond, D. C. (2022). Enrichment of the exocytosis protein STX4 in skeletal muscle remediates peripheral insulin resistance and alters mitochondrial dynamics via Drp1. Nat Commun, 13(1), 424. doi:10.1038/s41467-022-28061-w
- Merz, K. E., Tunduguru, R., Ahn, M., Salunkhe, V. A., Veluthakal, R., Hwang, J., . . . Thurmond, D. C. (2022). Changes in Skeletal Muscle PAK1 Levels Regulate Tissue Crosstalk to Impact Whole Body Glucose Homeostasis. Front Endocrinol (Lausanne), 13, 821849. doi:10.3389/fendo.2022.821849
- Stone, S. I., Wegner, D. J., Wambach, J. A., Cole, F. S., Urano, F., & Ornitz, D. M. (2020). Digenic Variants in the FGF21 Signaling Pathway Associated with Severe Insulin Resistance and Pseudoacromegaly. J Endocr Soc, 4(12), bvaa138. doi:10.1210/jendso/bvaa138
