EZT-KG10-1 Protocol.
Description
Cell to transfect:
Designations: KG-1
Cell Synonyms: CCL-246, human leukemia cell
Morphology: myeloblast
Growth properties: Suspension
Cellular products: HLA DR
Reverse transcriptase: negative
Antigen Expression: HLA A30, A31, B35, Cw4
Isoenzyme: AK-1, 0; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 1; PGM1, 1-2; PGM3, 0
Depositors: D.W. Golde
The KG-1 cell line was derived by H.P. Koeffler and D.W. Golde A bone marrow aspirate was obtained from a 59-year-old Caucasian male with erythroleukemia that evolved into acute myelogenous leukemia. KG-1 cells spontaneously differentiate to granulocyte and macrophage like cells.
They show a good response to colony stimulating factor(CSF).
The line is EBNA negative (EBNA-).
Cytogenetic analysis: The stemline chromosome
Features of The Transfection Reagent:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Specifically optimized to deliver nucleic acids into KG-1 cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Suitable for Reverse Transfection
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on KG-1 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for KG-1 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this KG-1 Cell Avalanche Transfection Reagent.
For Other Cells
KG-1 Cell Avalanche® Transfection Reagent (human leukemia cell) can also be used on the following cells with high transfection efficiencies.
Caki-1 Cell
293 Cell
293T/17 Cell
NIH/3T3 Cell
3T3-L1 Cell
CT26.WT Cell
NCTC Clone 929 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype |
Suspension |
| Cell Type |
Others |
| Disease |
Cancer |
| Names starting from |
K |
| Primary/Cell Line |
Cell Line |
| Product Sizes |
0.5 ml, 1.5 ml |
| Species |
Human |
| Tissue Sources |
Bone Marrow |
| Subcategories |
Cell Type/Cell Line Specific |
Documents
Protocols
MSDS
Citations or Feedback
