Jurkat-FHCRC Cell Avalanche® Transfection Reagent (human leukemia cell)

Name: Jurkat-FHCRC Cell Avalanche® Transfection Reagent (human leukemia cell)
SKU: EZT-JURK-1
Availability: InStock

Transfection of suspension cells, such as Jurkat-FHCRC cells, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments. However, electroporation usually leads to cell death, primarily when the electrical fields cause permanent permeabilization of the membrane and the consequent loss of cell homeostasis, in a process known as irreversible electroporation.

Jurkat-FHCRC Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Jurkat-FHCRC cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Jurkat-FHCRC cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

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SKU: EZT-JURK-1 Categories: All Cell Lines, Cell Type/Cell Line-specific Transfection Reagent, F-J, For Cell Lines, Transfection Reagents Tags: a weiss, cd3, Clone E601, homo sapiens, human leukemia cell, Jurkat, lymphoblast, male, T cell leukemia, t lymphocyte, TIB-152

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$714.00 – $1,264.00

Description

Cell to transfect:

Designations: Jurkat, Clone E6-1

Cell Synonyms: TIB-152, Clone E6-1, FHCRC, human leukemia cell

Depositors: A Weiss

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Disease: acute T cell leukemia

Cell Type: T lymphocyte;

Cellular Products: interleukin-2 (interleukin 2, IL-2)

Applications: transfection host

Receptors: T cell antigen receptor, expressed

Antigen Expression: CD3; Homo sapiens, expressed

Gender: male

This is a clone of the Jurkat-FHCRC cell line, a derivative of the Jurkat cell line. The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM.

Clone E6-1 cells produce large amounts of IL-2 after stimulation with phorbol esters and either lectins or monoclonal antibodies against the T3 antigen (both types of stimulants are needed to induce IL-2 production).

Features of The Transfection Reagent:

Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
Unique formulation-maximize transfection performance in Jurkat-FHCRC cells.
Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
0.5 ml is able to transfect about 1000 wells of 24-well plate
Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
Compatible with serum
Suitable for Reverse Transfection
Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on Jurkat-FHCRC cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for Jurkat-FHCRC cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Jurkat-FHCRC Cell Avalanche® Transfection Reagent.

For Other Cells

Jurkat-FHCRC Cell Avalanche® Transfection Reagent (human leukemia cell) can also be used on the following cells with high transfection efficiencies.

293 Cell

293T/17 Cell

DU 145 Cell

22Rv1 Cell

VCaP Cell

Capan-1 Cell

PANC-1 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight	0.5 lbs
Adherence Phenotype	Suspension
Cell Type	lymphocyte
Disease	Cancer
Names starting from	J
Primary/Cell Line	Cell Line
Product Sizes	0.5 ml, 1.5 ml
Species	Human
Tissue Sources	Blood
Subcategories	Cell Type/Cell Line Specific

Documents

Protocols

EZT-JURK-1 Protocol.

MSDS

EZT-JURK-1 MSDS

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