Human Urethra Epithelial Cell (HUEpC) Avalanche® Transfection Reagent

Transfection of primary cells, such as HUEpC, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments. However, electroporation usually leads to cell death, primarily when the electrical fields cause permanent permeabilization of the membrane and the consequent loss of cell homeostasis, in a process known as irreversible electroporation.

Human Urethra Epithelial Cell (HUEpC) Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HUEpC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HUEpC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

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$714.00$1,264.00

 
Description

Cells to transfect:

Cell Synonyms: HUEpC, Urethra Epithelial Cell

In addition to functioning as physical barriers against microbes, the urethral epithelial linings participate in the first line of host defense by production of a variety of proinflammatory mediators when exposed to microbes and microbial agents. This might occur via adhesion-mediated signalling by receptor interaction or via attachment-facilitated presentation of conserved microbial patterns to TLRs on epithelial cells.

Primary Human Urethra Epithelial Cell (HUEpC) is a good in vitro model for pathogenesis studies of urethral epithelial cells in various diseases.

Transfection reagent features:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Unique Formulation-Maximize transfection performance in HUEpC
  • Extremely gentle to cells
  • Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Single or multiple plasmids transfection
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines.
  • Compatible with serum
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human renal epithelial cell (HRE) after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human renal epithelial cell (HRE). This test result was confirmed with repeat experiments. The candidate formulas were also tested in human urethra epithelial cell (HUEpC) by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as Human Urethra Epithelial Cell (HUEpC) Avalanche® Transfection Reagent.

For Other Cells

Human Urethra Epithelial Cell (HUEpC) Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.

786-O Cell

Caki-1 Cell

MDCK Cell

Vero Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche® transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Adherent

Cell Type

Epithelial Cell

Disease

Healthy

Names starting from

H

Primary/Cell Line

Primary Cell

Product Sizes

0.5 ml, 1.5 ml

Species

Human

Tissue Sources

Urethra

Subcategories

Cell Type/Cell Line Specific

Documents

Citations or Feedback

Testimonials and Citations

 
Description

Cells to transfect:

Cell Synonyms: HUEpC, Urethra Epithelial Cell

In addition to functioning as physical barriers against microbes, the urethral epithelial linings participate in the first line of host defense by production of a variety of proinflammatory mediators when exposed to microbes and microbial agents. This might occur via adhesion-mediated signalling by receptor interaction or via attachment-facilitated presentation of conserved microbial patterns to TLRs on epithelial cells.

Primary Human Urethra Epithelial Cell (HUEpC) is a good in vitro model for pathogenesis studies of urethral epithelial cells in various diseases.

Transfection reagent features:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Unique Formulation-Maximize transfection performance in HUEpC
  • Extremely gentle to cells
  • Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Single or multiple plasmids transfection
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines.
  • Compatible with serum
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human renal epithelial cell (HRE) after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human renal epithelial cell (HRE). This test result was confirmed with repeat experiments. The candidate formulas were also tested in human urethra epithelial cell (HUEpC) by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as Human Urethra Epithelial Cell (HUEpC) Avalanche® Transfection Reagent.

For Other Cells

Human Urethra Epithelial Cell (HUEpC) Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.

786-O Cell

Caki-1 Cell

MDCK Cell

Vero Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche® transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Adherent

Cell Type

Epithelial Cell

Disease

Healthy

Names starting from

H

Primary/Cell Line

Primary Cell

Product Sizes

0.5 ml, 1.5 ml

Species

Human

Tissue Sources

Urethra

Subcategories

Cell Type/Cell Line Specific

Documents

Citations or Feedback

Testimonials and Citations

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