Description
Cells to transfect:
Cell Names: Human Skeletal Muscle Cell, Myoblast
Cell Synonyms: HSkMC, SkMC, HSMM, Skeletal Muscle
Human Skeletal Muscle Cells (HSkMC) usually are isolated from the limbal skeletal muscle. HSkMC can undergo differentiation to exhibit actin and myosin myofilaments. HSkMC have been used in the study of genetic muscular diseases such as Malignant Hyperthermia. HSkMC also have the potential to act as a cardiac graft, mending damage to the heart. Recently, cultured HSkMC have been used in micro gravity experiments to study the effects of low gravity environments on Human Skeletal Muscle.
Human Skeletal Muscle Myoblasts (HSMMs) usually are isolated from post-gestational tissue usually from the quadriceps or psoas tissue. HSMMs can be differentiated to form myotubes in culture.
Applications:
- Cellular development
- Differentiation
- Diabetes
- Insulin uptake or resistance
- Tissue repair
- Drug development
- Myotube development
- Co-culture with other cell types
The Transfection Reagent Features:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Specifically optimized to deliver nucleic acids into Human Skeletal Muscle Cells and Myoblasts
- Extremely gentle to cells
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human skeletal muscle cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human skeletal muscle cell. This test result was confirmed by transfecting human skeletal myoblasts mentioned above. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Skeletal Muscle Cell and Myoblast Avalanche® Transfection Reagent.
For Other Cells
Human Skeletal Muscle Cell and Myoblast (HSkMC, SkMC, HSMM) Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
Weight | 0.5 lbs |
---|---|
Adherence Phenotype |
Adherent |
Cell Type |
Skeletal Muscle Cell |
Disease |
Healthy |
Names starting from |
H |
Primary/Cell Line |
Primary Cell |
Product Sizes |
0.5 ml, 1.5 ml |
Species |
Human |
Tissue Sources |
Skeletal Muscle |
Subcategories |
Cell Type/Cell Line Specific |
Documents
Protocols
MSDS
Citations or Feedback
- Nasrin, F., Rahman, M. A., Masuda, A., Ohe, K., Takeda, J., & Ohno, K. (2014). HnRNP C, YB-1 and hnRNP L coordinately enhance skipping of human MUSK exon 10 to generate a Wnt-insensitive MuSK isoform. Sci Rep, 4, 6841. doi:10.1038/srep06841