EZT-HRE0-1 Protocol.
Description
Cells to transfect:
Cell name: Human Renal Epithelial cell
Cell Synonyms: HRE, Human Renal Proximal Tubele Epithelial Cell, RPTEC, HRPTEpC, HRCE, renal cortical epithelial cell
Human Renal Epithelial cells (HRE) have a pivotal role in kidney functions such as reabsorption of glucose and protein filtered by glomerulus and secretion of organic acids and drugs; they also play a part in the glucotoxicity associated with diabetes. The progression of renal dysfunction in type 2 diabetes heavily involves injury of the tubular epithelium as well as the glomerulus.
HRE provide a useful tool for studying various aspects of pathology and biology of the human renal proximal tubular cells in vitro. The cells can be used for the following applications:
- Renal physiology
- Cancer
- Cytokine production
- Glomerulonephritis
- Prostaglandin activity
- Toxicology
- Phagocytosis of immune complexes
- Cellular function and differentiation
Transfection reagent features
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Specifically optimized to deliver nucleic acids into HRE
- Highest efficiency to ensure experimental success
- Extremely gentle to cells
- Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Compatible with serum
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Developed and manufactured by EZ Biosystems
Data®
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human renal epithelial cell (HRE) after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human renal epithelial cell (HRE). This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Renal Epithelial cell (HRE) Avalanche Transfection Reagent.
For Other Cells
Human Renal Epithelial cell (HRE) Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype |
Adherent |
| Cell Type |
Epithelial Cell |
| Disease |
Healthy |
| Names starting from |
H |
| Primary/Cell Line |
Primary Cell |
| Product Sizes |
0.5 ml, 1.5 ml |
| Species |
Human |
| Tissue Sources |
Kidney |
| Subcategories |
Cell Type/Cell Line Specific |
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