EZT-PRSC-1 Protocol.
Description
Cells to transfect:
Cell name: Human Prostate Stromal Cell (PrSC)
Cell Synonyms: PrSC, Prostate Stromal Cell
Prostate cells provide a glandular function in the body by generating fluid which serves several functions in reproduction. Prostates consist of glandular epithelium embedded in a fibro-muscular stroma. The secretory luminal layer of the epithelium is made up of tall columnar cells that are responsible for the production of PSA, PAP and human kallikrein-2 that are secreted as part of the seminal fluid into the glandular lumina.
Human Prostate Stromal Cells (PrSC) are necessary for the normal growth and survival of epithelial cells in the prostate. A number of findings have suggested interactions between PrSC and the epithelium as well as importance of the extracellular matrix in prostate development and differentiation; alterations of these interactions may lead to prostate cancer progression (Djakiew et al., 1990; Cunha et al., 1996, 2002). Stromal®epithelial interactions appear to be reciprocal, mediated by steroid hormones, soluble and insoluble factors and their receptors, and constituents of the extracellular matrix (Chung, 1993; Rowley, 1999).
PrSC can be used for the following applications:
- Benign prostatic hyperplasia (BPH)
- Prostitis
- Prostate cancer
- Procreation research
- Drug discovery
- Male prostate physiology
Transfection reagent features:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Specifically optimized to deliver nucleic acids into PrSc
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
- synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human prostate stromal cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human prostate stromal cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Prostate Stromal Cell Avalanche Transfection Reagent.
For Other Cells
Human Prostate Stromal Cell (PrSC) Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype |
Adherent |
| Cell Type |
Stromal Cell |
| Disease |
Healthy |
| Names starting from |
H |
| Primary/Cell Line |
Primary Cell |
| Product Sizes |
0.5 ml, 1.5 ml |
| Species |
Human |
| Tissue Sources |
Prostate |
| Subcategories |
Cell Type/Cell Line Specific |
Documents
Protocols
MSDS
Citations or Feedback
- Liu, Y., Wang, R., Huang, R., Rutz, B., Ciotkowska, A., Tamalunas, A., . . . Hennenberg, M. (2023). Inhibition of growth and contraction in human prostate stromal cells by silencing of NUAK1 and -2, and by the presumed NUAK inhibitors HTH01-015 and WZ4003. Front Pharmacol, 14, 1105427. doi:10.3389/fphar.2023.1105427
- Wang, R., Schneider, S., Keppler, O. T., Li, B., Rutz, B., Ciotkowska, A., . . . Hennenberg, M. (2021). ADP Ribosylation Factor 6 Promotes Contraction and Proliferation, Suppresses Apoptosis and Is Specifically Inhibited by NAV2729 in Prostate Stromal Cells. Mol Pharmacol, 100(4), 356-371. doi:10.1124/molpharm.121.000304
- Yu, Q., Gratzke, C., Wang, R., Li, B., Kuppermann, P., Herlemann, A., . . . Hennenberg, M. (2019). A NAV2729-sensitive mechanism promotes adrenergic smooth muscle contraction and growth of stromal cells in the human prostate. J Biol Chem, 294(32), 12231-12249. doi:10.1074/jbc.RA119.007958
