EZT-HPEC-1 Protocol.
Description
Cells to transfect:
Cell Name: Human Prostate Epithelial Cell (HPrEpC)
Cell Synonyms: HPrEpC, Prostate Epithelial Cells, PrEC.
The prostate consists of glandular epithelium embedded in a fibro-muscular stroma. The epithelium is composed of two histologically distinct layers. The secretory luminal layer is made up of tall columnar cells that are responsible for the production of PSA, PAP and human kallikrein-2 that are secreted as part of the seminal fluid into the glandular lumina. This layer of cells is underpinned by a basal layer of cuboidal epithelial cells.
Human Prostate Epithelial Cells (HPrEpC) provide a useful tool for studying various aspects of pathology and biology of the human prostate epithelium in vitro. HPrEpC can be used for the following applications:
- Benign prostatic hyperplasia (BPH)
- Prostitis
- Prostate cancer
- Procreation research
- Drug discovery
- Male prostate physiology
Transfection reagent features:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Specifically optimized to deliver nucleic acids into HPrEpC
- Highest efficiency to ensure experimental success
- Extremely gentle to cells
- Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Compatible with serum
- Compatible with transfection in any plate formats
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human prostate epithelial cell (HPrEpC) after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human prostate epithelial cell (HPrEpC). This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Prostate Epithelial Cell (HPrEpC) Avalanche Transfection Reagent.
For Other Cells
Human Prostate Epithelial Cell (HPrEpC) Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype |
Adherent |
| Cell Type |
Epithelial Cell |
| Disease |
Healthy |
| Names starting from |
H |
| Primary/Cell Line |
Primary Cell |
| Product Sizes |
0.5 ml, 1.5 ml |
| Species |
Human |
| Tissue Sources |
Prostate |
| Subcategories |
Cell Type/Cell Line Specific |
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