Human Preadipocyte (HPAd) Avalanche® Transfection Reagent

Transfection of primary cells, such as Human Preadipocyte (HPAd), has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments. However, electroporation usually leads to cell death, primarily when the electrical fields cause permanent permeabilization of the membrane and the consequent loss of cell homeostasis, in a process known as irreversible electroporation.

Human Preadipocyte (HPAd) Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on HPAd. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HPAd. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Preadipocyte (HPAd) Avalanche® Transfection Reagent for transfection on HPAd. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

$714.00 – $1,264.00

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$714.00 – $1,264.00

 
Description

Cell to transfect:

Cell Name: Human Preadipocyte (HPAd)

Human Preadipocytes (HPAd) are derived from human adipose tissue. These fibroblast-like precursor cells can be differentiated into Human Adipocytes (HAd). HPAd derived HAd were used to study insulin-stimulated glucose transport, growth hormone enhanced lypolysis and obese gene expression. Significant differences in lipolysis and leptin production are observed in HPAd and HAd from human omental and subcutaneous adipose tissue. HPAd/HAd are a useful in vitro cellular model for investigating the causes and treatment of obesity, type II diabetes and cardiovascular diseases. Recently it was discovered that human adipocytes might be a new target for the immunodeficiency virus.

The Transfection Reagent Features:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Specifically optimized to deliver nucleic acids into HPAd
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human preadipocytes after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human preadipocytes. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Preadipocyte Avalanche Transfection Reagent.

For Other Cells

 

Human Preadipocyte (HPAd) Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.

3T3-L1

3T3-F442A

Ob17

AP-18

BJ Cell

Detroit 551 Cell

MG-63 Cell

786-O Cell

Caki-1 Cell

MDCK Cell

Vero Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype Adherent
Cell Type Preadipocyte
Disease Healthy
Names starting from H
Primary/Cell Line Primary Cell
Product Sizes 0.5 ml, 1.5 ml
Species Human
Tissue Sources Adipose
Subcategories Cell Type/Cell Line Specific

Documents


Protocols



PDF icon imageEZT-HPAD-1 Protocol.





MSDS



PDF icon imageEZT-HPAD-1 MSDS





Citations or Feedback

  • Chen, S., Swier, V. J., Boosani, C. S., Radwan, M. M., & Agrawal, D. K. (2016). Vitamin D Deficiency Accelerates Coronary Artery Disease Progression in Swine. Arterioscler Thromb Vasc Biol, 36(8), 1651-1659. doi:10.1161/atvbaha.116.307586 

Testimonials and Citations

 
Description

Cell to transfect:

Cell Name: Human Preadipocyte (HPAd)

Human Preadipocytes (HPAd) are derived from human adipose tissue. These fibroblast-like precursor cells can be differentiated into Human Adipocytes (HAd). HPAd derived HAd were used to study insulin-stimulated glucose transport, growth hormone enhanced lypolysis and obese gene expression. Significant differences in lipolysis and leptin production are observed in HPAd and HAd from human omental and subcutaneous adipose tissue. HPAd/HAd are a useful in vitro cellular model for investigating the causes and treatment of obesity, type II diabetes and cardiovascular diseases. Recently it was discovered that human adipocytes might be a new target for the immunodeficiency virus.

The Transfection Reagent Features:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Specifically optimized to deliver nucleic acids into HPAd
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human preadipocytes after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human preadipocytes. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Preadipocyte Avalanche Transfection Reagent.

For Other Cells

 

Human Preadipocyte (HPAd) Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.

3T3-L1

3T3-F442A

Ob17

AP-18

BJ Cell

Detroit 551 Cell

MG-63 Cell

786-O Cell

Caki-1 Cell

MDCK Cell

Vero Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype Adherent
Cell Type Preadipocyte
Disease Healthy
Names starting from H
Primary/Cell Line Primary Cell
Product Sizes 0.5 ml, 1.5 ml
Species Human
Tissue Sources Adipose
Subcategories Cell Type/Cell Line Specific

Documents


Protocols



PDF icon imageEZT-HPAD-1 Protocol.





MSDS



PDF icon imageEZT-HPAD-1 MSDS





Citations or Feedback

  • Chen, S., Swier, V. J., Boosani, C. S., Radwan, M. M., & Agrawal, D. K. (2016). Vitamin D Deficiency Accelerates Coronary Artery Disease Progression in Swine. Arterioscler Thromb Vasc Biol, 36(8), 1651-1659. doi:10.1161/atvbaha.116.307586 

Testimonials and Citations

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