EZT-HMSC-1 Protocol.
Description
Cell to transfect:
Cell Name: Human Mesenchymal Stem Cell (Human Marrow Stromal Cell) (hMSC)
Cell Synonyms: HMSC, Human Marrow Stromal Cell, Human Mesenchymal Stem Cell
Mesenchymal stem cells (hMSC) are usually harvested and cultured from normal human bone marrow. The cells have the ability to differentiate into osteogenic, chondrogenic and adipogenic lineages. Cells are positive for CD105, CD166, CD29, and CD44. Cells test negative for CD14, CD34 and CD45.
The potential of hMSC to maintain multipotency and proliferate extensively in vitro provides new avenues for cell-based therapy in the restoration of damaged or diseased tissue. Recent reports indicate that hMSC are capable of cell fates crossing germ layer boundaries. In addition to differentiating into multi-lineages of the mesoderm, these cells can also differentiate into neurons of ectodermal origin and hepatocyte-like cells of endodermal origin.
The Transfection Reagent Features:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Unique formulation-maximize transfection performance in hMSC.
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate
- synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human mesenchymal stem cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human mesenchymal stem cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Mesenchymal Stem Cell Avalanche Transfection Reagent.
FIG. 2. hMSC were transfected with GFP vector (pEGFP-N3) by using hMSC Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells
Human Mesenchymal Stem Cell (Human Marrow Stromal Cell) (hMSC) Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype |
Adherent |
| Cell Type |
Stem Cell |
| Disease |
Healthy |
| Names starting from |
H |
| Primary/Cell Line |
Primary Cell |
| Product Sizes |
0.5 ml, 1.5 ml |
| Species |
Human |
| Tissue Sources |
Bone Marrow |
| Subcategories |
Cell Type/Cell Line Specific |
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