Human Lung Microvascular Endothelial Cell (HLMVEC, HMVEC-L) Avalanche® Transfection Reagent

Transfection of primary cells, such as Human Lung Microvascular Endothelial Cell (HLMVEC, HMVEC-L), has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments. However, electroporation usually leads to cell death, primarily when the electrical fields cause permanent permeabilization of the membrane and the consequent loss of cell homeostasis, in a process known as irreversible electroporation.

Human Lung Microvascular Endothelial Cell (HLMVEC, HMVEC-L) Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HLMVEC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HLMVEC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

$714.00$1,264.00

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$714.00$1,264.00

 
Description

Cell to transfect:

Cell Name: Human Lung Microvascular Endothelial Cell (HLMVEC, HMVEC-L)

Cell Synonyms: : HLMVEC, HMVEC-L, HMVEC-LBl, and HMVEC-LLy

Human Lung Microvascular Endothelial Cells (HLMVEC) and elevated expression of chemoattractant cytokines are critical for directed migration of leukocytes into the lung during the induction and perpetuation of acute lung injury. HLMVEC provide a useful tool for studying various aspects of pathology and biology of the pulmonary microvasculature in vitro.

Applications:

  • Vascular pathology
  • Inflammation
  • Oncology
  • Pulmonary hypertension
  • Pulmonary disease/disorders
  • Drug toxicity

The Transfection Reagent Features:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Specifically optimized to deliver nucleic acids into HLMVEC
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human lung microvascular endothelial cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human lung microvascular endothelial cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Lung Microvascular Endothelial Cell Avalanche Transfection Reagent.

For Other Cells

Human Lung Microvascular Endothelial Cell (HLMVEC, HMVEC-L) Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.

786-O Cell

Caki-1 Cell

MDCK Cell

Vero Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Adherent

Cell Type

Endothelial Cell

Disease

Healthy

Names starting from

H

Primary/Cell Line

Primary Cell

Product Sizes

0.5 ml, 1.5 ml

Species

Human

Tissue Sources

Lung

Subcategories

Cell Type/Cell Line Specific

Documents



Protocols


MSDS



Citations or Feedback

Testimonials and Citations

 
Description

Cell to transfect:

Cell Name: Human Lung Microvascular Endothelial Cell (HLMVEC, HMVEC-L)

Cell Synonyms: : HLMVEC, HMVEC-L, HMVEC-LBl, and HMVEC-LLy

Human Lung Microvascular Endothelial Cells (HLMVEC) and elevated expression of chemoattractant cytokines are critical for directed migration of leukocytes into the lung during the induction and perpetuation of acute lung injury. HLMVEC provide a useful tool for studying various aspects of pathology and biology of the pulmonary microvasculature in vitro.

Applications:

  • Vascular pathology
  • Inflammation
  • Oncology
  • Pulmonary hypertension
  • Pulmonary disease/disorders
  • Drug toxicity

The Transfection Reagent Features:

  • Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
  • Specifically optimized to deliver nucleic acids into HLMVEC
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Developed and manufactured by EZ Biosystems

Data

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human lung microvascular endothelial cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human lung microvascular endothelial cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Lung Microvascular Endothelial Cell Avalanche Transfection Reagent.

For Other Cells

Human Lung Microvascular Endothelial Cell (HLMVEC, HMVEC-L) Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.

786-O Cell

Caki-1 Cell

MDCK Cell

Vero Cell

293 Cell

293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

Additional Information

Weight 0.5 lbs
Adherence Phenotype

Adherent

Cell Type

Endothelial Cell

Disease

Healthy

Names starting from

H

Primary/Cell Line

Primary Cell

Product Sizes

0.5 ml, 1.5 ml

Species

Human

Tissue Sources

Lung

Subcategories

Cell Type/Cell Line Specific

Documents



Protocols


MSDS



Citations or Feedback

Testimonials and Citations

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