EZT-HLMV-1 Protocol.
Description
Cell to transfect:
Cell Name: Human Lung Microvascular Endothelial Cell (HLMVEC, HMVEC-L)
Cell Synonyms: : HLMVEC, HMVEC-L, HMVEC-LBl, and HMVEC-LLy
Human Lung Microvascular Endothelial Cells (HLMVEC) and elevated expression of chemoattractant cytokines are critical for directed migration of leukocytes into the lung during the induction and perpetuation of acute lung injury. HLMVEC provide a useful tool for studying various aspects of pathology and biology of the pulmonary microvasculature in vitro.
Applications:
- Vascular pathology
- Inflammation
- Oncology
- Pulmonary hypertension
- Pulmonary disease/disorders
- Drug toxicity
The Transfection Reagent Features:
- Broad spectrum for the transfection of large plasmid, mRNA, siRNA, and/or other type of nucleic acids, which is best for co-transfection of different type and/or size of nucleic acids.
- Specifically optimized to deliver nucleic acids into HLMVEC
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- 0.5 ml is able to transfect about 1000 wells of 24-well plate
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Developed and manufactured by EZ Biosystems
Data
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human lung microvascular endothelial cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human lung microvascular endothelial cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Lung Microvascular Endothelial Cell Avalanche Transfection Reagent.
For Other Cells
Human Lung Microvascular Endothelial Cell (HLMVEC, HMVEC-L) Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Additional Information
| Weight | 0.5 lbs |
|---|---|
| Adherence Phenotype |
Adherent |
| Cell Type |
Endothelial Cell |
| Disease |
Healthy |
| Names starting from |
H |
| Primary/Cell Line |
Primary Cell |
| Product Sizes |
0.5 ml, 1.5 ml |
| Species |
Human |
| Tissue Sources |
Lung |
| Subcategories |
Cell Type/Cell Line Specific |
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